四种肝细胞系天然免疫相关分子的表达及HBV对其表达的影响  

Expression of the innate immune associated molecules in four hepatocyte lines and the influence of their expression by hepatitis B viral transfection

作　　者：刘敏[1] 郝友华[1] 丁红晖[1] 喻涛[1] 吴珺[1] 徐春利[1] 陆蒙吉[2,3] 杨东亮[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院临床免疫研究室,武汉市430030 [2]华中科技大学同济医学院附属同济医院病原生物学教研室,武汉市430030 [3]德国埃森大学医学院病毒学研究所

出　　处：《实用肝脏病杂志》2011年第1期11-14,20,共5页Journal of Practical Hepatology

基　　金：国家艾滋病和病毒性肝炎等重大传染病防治专项(No.2008ZX10002-011)

摘　　要：目的探讨四种肝细胞系Huh7、HepG2、HepG2.2.15和L02天然免疫相关分子的表达情况及HBV对其表达的影响。方法提取Huh7、HepG2、HepG2.2.15和L02四种肝细胞及转染后Huh7、HepG2和HepG2.2.15细胞mRNA,逆转录合成cDNA,采用PCR法检测天然免疫相关分子的表达情况。结果四种细胞均能表达TLRs家族的TLR1、TLR3、TLR4、TLR9,NLRs家族的NOD1和RLRs家族的RIG1等天然免疫相关分子;HepG2和Huh7转染HBV可复制性克隆后出现MDA5和A20的表达下调。结论四种肝细胞系均能表达一系列天然免疫相关分子,不同肝细胞系间表达存在一定的差异。HBV瞬时转染和稳定表达能不同程度地影响这些天然免疫相关分子的表达水平。Objective To investigate the expression of innate immune-associated molecules in Huh7,HepG2,HepG2.2.15 and L02 hepatocyte lines and the influence of their expression by hepatitis B viral transfection in intro.Methods The total mRNA of the four cultured cells and three transfected cells by HBV replication clone（pAAV-HBV1.2）were extracted and reverse transcripted to cDNA.The expression of the innate immune-associated molecules such as TLRs,NLRs and RLRs were detected by PCR.Results The results showed that all of the four cell lines not only expressed the TLRs such as TLR1,TLR3,TLR4 and TLR9,but also expressed the NLRs such as NOD1,and RLRs such as RIG1.The expression of MDA5 and A20 decreased in transfected HepG2 and Huh7 cells.Conclusion Hepatocytes can express a series of innate immune-associated molecules such as TLRs and RLRs.There are distinct expression in different hepatocyte lines.HBV could influence the expression of the innate immune-associated molecules in hepatocyte lines.

关 键 词：肝细胞系 天然免疫相关分子 乙型肝炎病毒 

分 类 号：R512.62[医药卫生—内科学]