红细胞调控CD4/CD8的血细胞快速培养实验研究  

Red Blood Cells Can Regulate CD4/CD8 in the Blood Cells Culture

作　　者：郭峰[1] 钱宝华[1] 张乐之[2] 张军[2] 

机构地区：[1]第二军医大学长海医院输血科血液免疫研究室,上海200433 [2]第二军医大学长海医院实验诊断科,上海200433

出　　处：《临床医学工程》2011年第1期10-12,共3页Clinical Medicine & Engineering

基　　金：国家自然科学基金项目"现代系统免疫学自然与分离体系实验研究"(批准号30671940)

摘　　要：目的利用系统免疫学血细胞培养系统模式,在体外研究红细胞调控CD4/CD8规律及其意义。方法 22例人枸橼酸抗凝新鲜血(11例正常人和11例原发性肝癌患者)。全血细胞培养组(自然组):0.2ml全血细胞悬液加0.3ml血浆和0.2ml生理盐水,白细胞培养组(分离组):0.2ml白细胞悬液加0.3ml血浆和0.2ml生理盐水,充分混匀,37℃温育1小时,2000转/分离心5分钟,两管的白细胞CD4和CD8表达水平用PC-5酶标抗CD4单抗和FITC-酶标抗CD8单抗测定,红细胞调节率=(全血细胞培养组CD4或CD8或CD4/CD8水平-白细胞培养组CD4或CD8或CD4/CD8水平)÷白细胞培养组CD4或CD8或CD4/CD8水平。结果 22例全血细胞培养组CD4/CD8水平(1.40±0.59)明显高于白细胞培养组(0.48±0.26),t=6.6918,P<0.01,正常人的白细胞培养组CD4/CD8水平(0.58±0.27)明显高于肝癌的白细胞培养组CD4/CD8水平(0.35±0.19,P<0.01),正常人CD4/CD8调节率(2.42±2.39)高于肝癌患者CD4/CD8调节率(2.07±1.27)。结论本实验研究属于系统免疫学和计算免疫学领域,正常人红细胞能高调节CD4/CD8水平,在原发性肝癌中,细胞免疫功能低下,表现CD4/CD8表达低水平与红细胞对CD4/CD8低调节率密切相关。Objective Using systems immunology method of rapid blood culture system,we have studied red blood cells to regulate the levels of CD4/CD8 in vitro.Method Whole blood cells culture group：0.2 ml of whole blood cells suspension and 0.3 ml of auto-plasma and 0.2 ml of saline;white blood cells culture group：0.2 ml of white blood cells suspension and 0.3 ml of auto-plasma and 0.2 ml of saline,mixed fully,and incubated at 37 ℃ for 60 minutes,then were centrifuged at 2 000 rounds per minute for 5 minutes.Expression level of CD4 and CD8 on white blood cells in two tube bottom mixture was determined by FITC-Conjugated Abs（CD8） and PC-5-conjugated Abs（CD4）.Regulation rate of red blood cells =（level of CD4 or CD8 or CD4/CD8 in whole blood cells culture-level of CD4 or CD4/CD8 in white blood cells culture） ÷level of CD4 or CD8 or CCD4/CD88 in white blood cells culture.Result The level（1.40 ± 0.19） of CD4/CD8 in whole blood cells culture was significantly higher than that（0.48 ± 0.26） of CD4/CD8 in white blood cells culture（n=22,t=6.6918,P 0.01）.The level（0.58 ± 0.27） of CD4/CD8 in white blood cells culture of normal people was significantly higher than that（0.35 ± 0.19） of CD4/CD8 in white blood cells culture of patient whit primary hepatic cancer（n=11,t=4.4003,P 0.01）,CD4/CD8 regulation rate（2.42 ± 2.39） in normal people was higher than that（2.07 ± 1.27） CD4/CD8 regulation rate in patient with P.H.C.Conclusion The experimental study is a systems immunology and computational immunology.Red blood cells can regulate level of CD4/CD8.In patients with primary hepatic cancer,there is a close relationship between low levels of CD4/CD8 and low CD4 /CD8 regulate rate of red blood cells.

关 键 词：系统免疫学 原发性肝癌 CD4/CD8 红细胞吸附率 血细胞培养系统模式 

分 类 号：R392-33[医药卫生—免疫学] R446.11[医药卫生—基础医学]