大鼠全脑缺血缺氧损伤后血液中S-100、CK-BB、NSE水平变化的研究  

A Study on the Changes of S-100,CK-BB and NSE Levels in Blood after Hypoxic-ischemic Brain Damage in Rats

作　　者：金泓[1,2] 赖旭东[1,2] 钟莉[1,3] 

机构地区：[1]暨南大学第四附属医院 [2]广州市红十字会医院急诊科,广东广州510220 [3]广州市红十字会医院检验科,广东广州510220

出　　处：《临床医学工程》2011年第1期19-20,共2页Clinical Medicine & Engineering

摘　　要：目的探讨大鼠全脑缺血缺氧损伤后血液中S-100蛋白(S-100)、磷酸肌酸激酶脑型同工酶(CK-BB)、神经元特异性烯醇化酶(NSE)的水平变化。方法采用成年SD大鼠全脑缺血缺氧损伤模型:戊巴比妥腹腔注射麻醉后,用4.0双线结扎右侧颈总动脉,恢复4小时后,在37℃恒温低氧箱内,给予吸入8%氧气和92%氮气混合气体2小时。用放射免疫法和琼脂糖凝胶电泳法对对照组、缺氧缺血后6、12、24、48、72小时进行动态观测全脑缺血后血液中S-100、CK-BB、NSE水平的变化。结果在全脑缺血后,与对照组比较,血液和脑脊液中S-100、CK-BB、NSE在24～48小时都有明显增加(P<0.05)。结论在全脑缺血损伤时血液和脑脊液中S-100、CK-BB、NSE水平都在早期明显升高,提示这三种指标可作为评价大鼠全脑缺血缺氧损伤的早期诊断指标。Objective To analyse the changes of S-100 protein （S-100）, creatine phosphokinase BB isoenzyme （CK-BB）, and neuron-specific enolase （NSE） levels in blood after hypoxic-ischemic brain damage in rats. Methods The model ofhypoxic-ischemic brain damage in postnatal 180 days SD rats. The model was established by ligation of the right common carotid arteries for 4 hrs in rats, and then expo- sure of the rats to 8% of oxygen and 92% of nitrogen for 2 hrs. The blood samples were taken from 90 rats at the sequential time points of 6, 12, 24, 48, 72 hours after the model was established. Twenty-four normal rats were used as control. The dynamic changes of S-100, CK-BB and NSE were detected. Results All three biochemical markers significantly increased in blood and CSF of experimental rats when compared to controls. The peak level of S-100, CK-BB and NSE occured at 12 hrs after hypoxic-ischemic injury in blood （P 〈0.05）. Conclusion The most obvious changes of S-100, CK-BB, NSE in hypoxic-ischemic injury rats were seen at 12 - 24 hours, the three biochemical markers might be helpful in the early diagnosis of hypoxic-ischemic cerebral injury.

关 键 词：大鼠 脑缺血 磷酸丙酮酸水合酶 神经组织蛋白质S-100 肌酸激酶同工酶类 

分 类 号：R651.15[医药卫生—外科学]