不同产地黄柏及关黄柏有效成分的含量测定  

Determinations of Active Compositions in Cortex Phellodendri Chinensis and Cortex Phellodendri Amurensis from Different Areas by HPLC

作　　者：朱志明[1] 赖潇潇[1] 苏慕霞[2] 

机构地区：[1]广东省中医院药学部,广东广州510120 [2]广州中医药大学中药学院,广东广州510006

出　　处：《临床医学工程》2011年第1期106-108,共3页Clinical Medicine & Engineering

摘　　要：目的考察不同产地黄柏和关黄柏有效成分的含量,为这两个品种质量标准提供一定的依据。方法采用HPLC测定不同产地黄柏和关黄柏中盐酸小檗碱、盐酸药根碱和盐酸巴马汀的含量,以kromasil C18(150mm×4.6mm5μm)为色谱柱;流动相:乙腈-0.1%磷酸溶液(50:50)(每100mL加十二烷基硫酸钠0.1g);流速:1.0mL/min;柱温:25℃;检验波长:265nm。结果结果表明,川黄柏中以盐酸小檗碱的含量最高,为3.73%～6.131%之间,药根碱和巴马汀的含量较低,分别在0.007%～0.061%和0.027%～0.082%之间;关黄柏中盐酸小檗碱的含量在0.474%～1.240%之间,巴马汀的含量在0.516%～1.224%之间,以药根碱的含量最低,在0.008%～0.043%之间。结论不同产地的黄柏及关黄柏的含量差异较大,两个品种的含量差异也较大。Objective To determine the contents of active compositions of in different areas of Cortex Phellodendri Chinensis and Cortex Phellodendri Amurensis,to provide some academic basis for quality criterion of Cortex Phellodendri Chinensis.Methods The method of HPLC was used to determine the content of berberine hydrochloride,palmatine hydrochloride and jatrorrhizine hydrochloride in different areas of Cortex Phellodendri Chinensis and Cortex Phellodendri Amurensis.The method was established on a Kromasil C18 column with the mobile phase consisting of acetonitril-0.1% phosphonic acid（50：50）（per 100 mL plus sodium lauryl sulfate 0.1 g） and flow rate of 1.0 mL/min and the temperature at 30 ℃,the detection wavelength was 265 nm.Results The results showed that the highest content was berberine hydrochloride in Cortex Phellodendri Chinensis,between 3.73% ～ 6.131%;the content of jatrorrhizine and palmatine were low,between 0.007% ～ 0.061% and 0.027% ～ 0.082%,respectively;the lowest content was jatrorrhizine in Cortex Phellodendri Amurensis,between 0.008% ～ 0.043%,berberine hydrochloride between 0.474% ～ 1.224%,palmatine between 0.516% ～ 1.224%.Conclusion The content of active compositions was significantly different in different areas of Cortex Phellodendri Chinensis and Cortex Phellodendri Amurensis,the content of the two species was quite different.

关 键 词：黄柏 关黄柏 HPLC 盐酸小檗碱 盐酸药根碱 盐酸巴马汀 

分 类 号：R282.703[医药卫生—中药学]