高效液相色谱法测定左旋多巴片的有关物质  

Determination of related substances in levodopa tablet by HPLC

作　　者：赵雷[1] 

机构地区：[1]浙江大学医学院附属儿童医院,浙江杭州310003

出　　处：《中国当代医药》2011年第2期43-44,共2页China Modern Medicine

摘　　要：目的:采用高效液相色谱法测定左旋多巴片的有关物质。方法:采用依利特Spherisorb C18柱(5μm,150×4.6mm),以0.01mol/L磷酸二氢钾缓冲液(含0.003mol/L辛烷磺酸钠,pH3.0)-甲醇(84:16)为流动相,检测波长280nm,流速1.0ml/min,柱温30℃。结果:降解产物在该色谱条件下与左旋多巴分离良好。结论:本方法用于测定左旋多巴片有关物质,方法简便,快速,结果准确。Objective：To use an RP-HPLC method for determination of related substances in levodopa.Methods：The method was performed on Spherisorb column（5μm,150×4.6mm）,the mobile phase A was 0.01mol/L KDP buffer （contain 0.003mol/L Octane-1-sulfonic acid sodium salt,pH3.0）,the mobile phase B was MeOH,the detection wavelength was 280nm.The flow rate was 1.0ml/min.Results：The related substances in levodopa were separated successfull.Conclusion：The Method is simple,accurate and good reproducibility.

关 键 词：左旋多巴片 高效液相色谱法 有关物质 

分 类 号：R927.11[医药卫生—药学]