樟树褐斑病病原形态及分子鉴定  

Morphological and molecular identification of brown spot of Cinnamomum camphora

作　　者：王明生[1] 吴小芹[1] 丁晓磊[1] 王焱 马凤林 叶建仁[1] 

机构地区：[1]南京林业大学森林资源与环境学院,南京210037 [2]上海市林业总站

出　　处：《林业科技开发》2011年第1期63-65,共3页China Forestry Science and Technology

基　　金：上海市绿化局项目"上海绿化林业病虫害预警体系与防治关键技术研究"(编号:ZX061210)

摘　　要：樟树褐斑病是一种危害幼龄樟树枝干部的樟树病害,发生于上海宝山地区。经过调查研究,发现这种病害主要从伤口侵入,危害枝干的韧皮部,引起幼龄樟树的枯死。通过形态学和分子生物学相结合的方法,对这种枝干病害的病原进行了鉴定,结果为芒果球座菌(Guignardia mangiferae)。该菌菌落橄榄绿色,在PDA培养基上可形成有性和无性阶段,产生近椭圆形的分生孢子和梭形子囊孢子。利用ITS区序列可以区分其和柑桔黑斑病菌(G.citricarpa)。Cinnamomum camphora Brown spot often damaged branch and trunk of seedlings,which happened in Shanghai suburb Baoshan area.Through investigation and study,we found that the pathogen could invade host by wound,damage phloem of stems and cause the seedlings dead;According to morphologic and molecular biological characterization,the pathogen of this disease was identified as Guignardia mangiferae,its colony was olive green,the characterizations of sexual and asexual generation appeared on PDA medium,they were nearly elliptic conidia and fusiform ascospores;G.mangiferae and Guignardia citricarpa can be differentiated by ITS sequences.

关 键 词：樟树 芒果球座菌 形态学 分子鉴定 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治]