白蜡无性系硬枝扦插繁殖技术  

Study on hardwood cutting propagation of Fraxinus chinensis clones

作　　者：张晓文[1] 曹帮华[1] 洪丕征[1] 张玉娟[1] 秦永建[2] 

机构地区：[1]山东农业大学农业生态重点实验室,山东泰安271000 [2]山东省泰安市岱岳区林业局

出　　处：《林业科技开发》2011年第1期102-105,共4页China Forestry Science and Technology

基　　金：山东省农业良种工程项目(编号:鲁科农字[2006]90号)

摘　　要：以1年生白蜡无性系枝条在不同插穗株龄、基质、生长素种类、生长素浓度及处理时间4个因素进行L9（34）正交试验,研究不同条件下白蜡扦插繁殖技术及生根过程中重要生根关联酶吲哚乙酸氧化酶（IAAO）、多酚氧化酶（PPO）和过氧化物酶（POD）的活性动态变化。结果表明9个处理组合中,以处理9（5~6年生雄株1年生插穗、纯沙＋纯珍珠岩＋蛭石混合基质、IAA、100 mg/L、处理2 h）最优。白蜡扦插生根过程可分为愈伤形成期、不定根诱导期和不定根伸长期3个阶段,在生根过程中3种生根关联酶活性变化呈现规律性,不同处理间和各阶段3种酶活性差异显著,说明在白蜡扦插生根过程中,不定根的发生和伸长与这3种生根关联酶活性有密切联系。The research used the 1-year-old branch cutting of Fraxinus chinensis clones under four different treatment： age of cuttings,media,types of auxin,auxin concentration and treatment time to conduct the L9（34） orthogonal experiment and study the technology on hardwood cutting propagation of F.chinensis clones and the activity dynamic variation of important rooting enzyme： indole acetic acid oxidase（IAAO）,polyphenol oxidase（PPO） and peroxidase（POD）.The results showed that： in the 9 treatments tested,the 9th treatment（1-year-old cutting from 5~6 year-old male plant,mixed medium of pure sand,pure perlite and vermiculite IAA,100 mg/L,2 h treatment） is the best.The rooting process of F.chinensis cutting propagation can be divided into 3 periods： callus formation,adventitious root induction,dventitious root elongation.There was regular change of the three rooting-related enzymes during rooting period.The activity of the 3 rooting enzymes is clearly distinguished in different treatments and rooting phases,indicating that in the rooting process of F.chinensis cutting,the occurrence and elongation of adventitious roots are closely linked with these 3 rooting enzymes＇activity.

关 键 词：白蜡 扦插繁殖 生根关联酶 酶活性变化 

分 类 号：S685.12[农业科学—观赏园艺]