温度、光照及杀菌剂对板栗疫病菌生长的影响  

Effects of Different Temperatures,Light Conditions and Fungicides on Growth of Cryphonectria parasitica Mycelial

作　　者：吴群[1] 程中平[2,3] 黄俊斌[4] 胡春根[1] 王章利[2] 

机构地区：[1]华中农业大学园艺林学学院,武汉430070 [2]中国科学院武汉植物园 [3]中国科学院植物种质创新与特色农业重点实验室 [4]华中农业大学植物科学技术学院

出　　处：《中国南方果树》2011年第1期20-24,共5页South China Fruits

基　　金：武汉市科技攻关项目(200920322141);中国科学院重要方向性项目(KSCX2-YW-N-032)资助

摘　　要：从湖北省板栗主要产区具典型板栗疫病症状枝条上分离得到3个类型菌株:JS-6、TC-4和LT-1,根据形态学特征和分子鉴定结果,确定这些菌株的病菌均为Cryphonectria parasitica。以PDA为培养基对这些菌株进行不同温度(4～35℃)和光照条件的培养试验结果表明,菌丝生长最适温度为30℃,菌丝在光暗交替和光照条件下的生长速度较黑暗条件下快;还测得病菌的致死温度为50℃。以井冈霉素、万兴乳油、多菌灵可湿性粉剂、代森锌超微可湿性粉剂、世高水分散粒剂等杀菌剂进行抑杀板栗疫病菌生长试验结果表明,5种杀菌剂在供试浓度下对病菌生长都有一定的抑制作用,半效应浓度EC50以50%多菌灵可湿性粉剂剂量最小,10%世高水分散粒剂和5%井冈霉素可溶性粉剂其次。Three strains,JS-6,TC-4 and LT-1,were isolated from chestnut blight disease infected branches of trees in the main chestnut production areas in Hubei.Morphological and molecular analysis identified all the three strains were Cryphonectria parasitica.The isolates were cultivated on PDA（potato-dextrose agar） culture medium and incubated in different temperatures（from 4 ℃ to 35 ℃ with 5 ℃ gradient） under different light conditions.Anti-mycelia experiment was carried out with 5 fungicides（Jinggangmycin,Charisma EC,Carbendazim,Dithane and Shigao）.The results indicated that the optimal temperature for mycelia growth was 30 ℃ and that the lethal temperature was 50 ℃;mycelia growth was quicker under weak light or light-dark alternation conditions;all five fungicides showed inhibitory effects on mycelia growth to some extent.The lowest dosage reaching the median effect concentration（EC50）was 50% Carbendazim followed by 10% Shigao and 50% Jinggangmycin,whereas the highest dosage reaching EC50 was 20.67% charisma.

关 键 词：板栗疫病菌 温度 光照 杀菌剂 

分 类 号：S436.64[农业科学—农业昆虫与害虫防治]