高效液相色谱法测定生物黏附剂中辛苯醇醚含量  

Determination of Octoxynol-9 in Biological Adhesive by HPLC

作　　者：刘苗苗[1] 赵齐[2] 沈丽琳[1] 

机构地区：[1]天津市计划生育研究所,天津300191 [2]天津大学化学工程联合国家重点实验室,天津300072

出　　处：《中国药业》2011年第3期14-15,共2页China Pharmaceuticals

摘　　要：目的建立测定外用生物黏附剂中辛苯醇醚含量的高效液相色谱法。方法采用岛津VP-ODS型色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(90∶10),流速1.0mL/min,检测波长276 nm。结果辛苯醇醚进样量在1.02～24.48μg范围内与峰面积线性关系良好(r=0.999 6),平均回收率为99.06%,RSD为1.14%(n=6)。结论高效液相色谱法灵敏、准确、快速,重现性好,可用于外用生物黏附剂中辛苯醇醚的含量测定。Objective To establish a content determination method of octoxynol-9 in biological adhesive for external use by HPLC.Methods The Shimadzu VP-ODS chromatographic column（250 mm×4.6 mm,5 μm） was adopted with the mobile phase of methanol-water（90 ∶10） and the flow rate of 1.0 mL/min.The detecting wavelength was 276 nm.Results The calibration curves were linear within the range of 1.02-24.48 μg（r=0.999 6）.The average recovery rate was 99.06%,RSD was 1.14%（n=6）.Conclusion The method is sensitive,accurate,rapid and reproducible,which can be used for the determination of octoxynol-9 in biological adhesive for external use.

关 键 词：高效液相色谱法 生物黏附剂 辛苯醇醚 含量测定 

分 类 号：R927.2[医药卫生—药学] R979.2