Molecular Characterization of Segments S7 to S10 of a Southern Rice Black-streaked Dwarf Virus Isolate from Maize in Northern China  

作　　者：Xiao Yin Fei-fei Xu Fang-qiang Zheng Xiang-dong LI Bao-shen Liu Chun-qing Zhang 

机构地区：[1]Shandong Key Laboratory for Agricultural Microbiology, College of Plant Protection, Shandong Agricultural University, Tai＇an, Shandong 271018, China [2]College of Agronomy, Shandong Agricultural University, Tai＇an, Shandong 271018, China

出　　处：《Virologica Sinica》2011年第1期47-53,共7页中国病毒学（英文版）

基　　金：National Natural Science Foundation of China (30971895, 31011130031);Special Research Funds for the Doctoral Program of Higher Education (20080434006);Grants from Ministry of Science and Technology (2009ZX08003-014B);Shandong province(2009GG10009021);Modern maize industrial system of Shandong province

摘　　要：Southern rice black-streaked dwarf virus (SRBSDV) is a novel Fijivirus prevalent in rice in southern and central China,and northern Vietnam. Its genome has 10 segments of double-stranded RNA named S1 to S10 according to their size. An isolate of SRBSDV,JNi4,was obtained from naturally infected maize plants from Ji'ning,Shandong province,in the 2008 maize season. Segments S7 to S10 of JNi4 share nucleotide identities of 72.6%-73.1%,72.3%-73%,73.9%-74.5% and 77.3%-79%,respectively,with corresponding segments of Rice black-streaked dwarf virus isolates,and identities of 99.7%,99.1%-99.7%,98.9%-99.5%,and 98.6%-99.2% with those of SRBSDV isolates HN and GD. JNi4 forms a separate branch with GD and HN in the phylogenetic trees constructed with genomic sequences of S7 to S10. These results confirm the proposed taxonomic status of SRBSDV as a distinct species of the genus Fijivirus and indicate that JNi4 is an isolate of SRBSDV. Shandong is so far the northernmost region where SRBSDV is found in China.Southern rice black-streaked dwarf virus （SRBSDV） is a novel Fijivirus prevalent in rice in southern and central China,and northern Vietnam. Its genome has 10 segments of double-stranded RNA named S1 to S10 according to their size. An isolate of SRBSDV,JNi4,was obtained from naturally infected maize plants from Ji＇ning,Shandong province,in the 2008 maize season. Segments S7 to S10 of JNi4 share nucleotide identities of 72.6%-73.1%,72.3%-73%,73.9%-74.5% and 77.3%-79%,respectively,with corresponding segments of Rice black-streaked dwarf virus isolates,and identities of 99.7%,99.1%-99.7%,98.9%-99.5%,and 98.6%-99.2% with those of SRBSDV isolates HN and GD. JNi4 forms a separate branch with GD and HN in the phylogenetic trees constructed with genomic sequences of S7 to S10. These results confirm the proposed taxonomic status of SRBSDV as a distinct species of the genus Fijivirus and indicate that JNi4 is an isolate of SRBSDV. Shandong is so far the northernmost region where SRBSDV is found in China.

关 键 词：Southern rice black-streaked dwarf virus （SRBSDV） Maize rough dwarf disease （MRD） Identity Phylogenetic analysis 

分 类 号：Q987[生物学—遗传学] S435.111.4[生物学—人类学]