天麻药材高效液相色谱指纹图谱研究  

HPLC Fingerprint of Gastrodia elata Blume

作　　者：黄小玲[1] 杨春燕[1] 袁慧文[2] 

机构地区：[1]广东省深圳市慢性病防治中心,广东深圳518020 [2]广东省深圳市药品检验所,广东深圳518029

出　　处：《中国药业》2011年第2期17-19,共3页China Pharmaceuticals

摘　　要：目的研究天麻药材的高效液相色谱指纹图谱,为其质量控制提供可靠的方法。方法采用高效液相色谱法。色谱柱为Ultimate XB-C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-1%冰醋酸水溶液(梯度洗脱),柱温为30℃,流速1 mL/min,检测波长270 nm。结果初步建立了天麻药材的高效液相色谱指纹图谱,标定出16个共有峰;天麻素的加样加收率为102.66%,RSD<2.5%(n=6)。结论高效液相色谱指纹图谱为天麻药材质量控制提供了一种新方法,并为未知天麻样品的鉴别提供一个准确、快捷的工具。Objective To study the HPLC fingerprint of medicinal material Gastro dia elata Blume to provide the reliable method for its quality control.Methods T he analysis was performed on Ultimate XB-C18 column（250 mm×4.6 mm,5 μm） with the mobile phase of methanal-1%glacial acetic acid aqueous solution in the gr adient elution.The column temperature was 30 ℃and the flow rate was 1.0 mL/min. The UV detector was set at 270 nm.Results The HPLC fingerprint of Gastrodia elat a Blume was preliminarily established and 16 common peaks were displayed in the fingerprint.The average sample recovery rate of gastrodine was 102.66%,RSD 2. 5%（n=6）.Conclusion The HPLC fingerprint may provide a new model for controlling the quality of medicinal material Gastrodia elata Blume and also may provide an accurate and rapid tool for identifying unknown sample of Gastrodia elata Blume.

关 键 词：天麻 高效液相色谱法 指纹图谱 质量控制 天麻素 

分 类 号：R284.1[医药卫生—中药学] R282.71[医药卫生—中医学]