白菜型油菜高效离体再生体系的建立  

Establishment of Effective Regeneration System of Brassica rapa in vitro

作　　者：裴冬丽[1,2] 

机构地区：[1]商丘师范学院生命科学系,植物与微生物互作重点实验室,河南商丘476003 [2]河南农业大学农学院,河南郑州450002

出　　处：《北方园艺》2011年第1期127-129,共3页Northern Horticulture

基　　金：河南省科技发展计划国际合作资助项目(084300510076);河南省科技厅基础与前沿技术资助项目(082300430320)

摘　　要：以白菜型油菜(Brassicarapa)的亚种菜心(Brassica parachinensis)的子叶和下胚轴为外植体,研究不同浓度的NAA和6-BA配比对菜心组织培养的影响。结果表明:菜心子叶和下胚轴组织培养的再生最佳培养基分别是MS+6-BA1.5 mg/L+NAA0.2 mg/L+AgNO35 mg/L和MS+6-BA2.0 mg/L+NAA0.2 mg/L+AgNO35 mg/L;不定芽生根最佳培养基是MS+NAA0.2 mg/L。同时还研究了AgNO3浓度对菜心组织培养的影响,在培养基中附加AgNO3能提高菜心芽的诱导频率。Cotyledons and hypocotyls of Brassica parachinensis subspecies of Brassica rapa were used as explants to study the effect of different concentration ratio of NAA and 6-BA on Brassica parachinensis tissue culture.The results showed that the best regeneration medium for cotyledons and hypocotyls of Brassica parachinensis were MS＋6-BA 1.5 mg/L＋NAA 0.2 mg/L＋AgNO3 5 mg/L and MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L＋AgNO3 5 mg/L respectively.The best root medium of adventitious buds was MS＋NAA 0.2 mg/L.It was also discussed the influence of the concentration of AgNO3 on Brassica parachinensis tissue culture.The high inducement frequency of shoot regeneration was gained by addition of AgNO3 in the medium.

关 键 词：白菜型油菜 植株再生 组织培养 

分 类 号：S634.3[农业科学—蔬菜学]