树莓果实总RNA提取方法的比较研究  

Study on Isolation of Total RNA from Raspberry Fruit

作　　者：刘丹[1] 高庆玉[1] 张丙秀[1] 焦奎宝[2] 

机构地区：[1]东北农业大学园艺学院,黑龙江哈尔滨150030 [2]黑龙江省农业科学院浆果所,黑龙江绥棱152204

出　　处：《北方园艺》2011年第1期136-138,共3页Northern Horticulture

基　　金：国家公益性行业农业科研专项基金资助项目(ny-hyzx07-028)

摘　　要：比较了SDS法和Trizol法提取树莓果实总RNA的效果。结果表明:Trizol法提取的RNA经电泳检测,未见条带,说明Trizol法并不适合富含多糖多酚的树莓。SDS方法提取树莓果实总RNA完整性好,28S和18S条带清晰,OD260/OD280值为1.83,在1.7～2.0之间,OD260/OD230值为2.01。结果证明,SDS法提取的总RNA可直接用于分子克隆等分子生物学实验。SDS and Trizol were compared for extracting total RNA from fruit of raspberry in this paper.The results showed that no bands of RNA isolated by Trizol were found by agarose gel electrophoresis,indicating Trizol method was not suitable for the raspberry rich in polysaccharides and polyphenols.Total RNA isolated by the method of SDS displayed a good integrity,with clear bands of 28S and 18S,OD260/OD280 was 1.83 between 1.7 and 2.0,and OD260/OD230 was 2.01.Reverse transcription results showed that total RNA extracted by the SDS can be directly used for molecular cloning and other molecular biology experiments.

关 键 词：树莓 总RNA SDS法 TRIZOL法 

分 类 号：S663.2[农业科学—果树学]