FMDV P1基因在重组PRV的表达及实验免疫研究  

FMDV P1 gene expression in recombinant PRV and experimental immunization

作　　者：史秋梅[1,2,3] 高桂生[1] 向华[2] 李敏[1,3] 宣华[1,3] 

机构地区：[1]广东省农业科学院兽医研究所,广东广州510640 [2]河北科技师范学院动物科学系,河北省预防兽医学重点实验室,河北昌黎066600 [3]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2011年第2期156-160,共5页Chinese Journal of Veterinary Science

基　　金：国家"十一五"科技支撑项目(2006BAD06A17);广东省科技攻关项目(2005A20902002);广东省社会发展规划项目(2005B2081002)

摘　　要：为获得FMDV的衣壳蛋白前体P1和P1与3C基因的重组伪狂犬病毒,并评价两重组病毒的免疫效果,进行了本项研究。将构建的重组转移质粒pIESZP1和pUTK3CP1,用脂质体转染预先感染0.1 MOI PRV的Vero细胞,经120 h培养收获病毒后,再分别用蓝斑筛选和BrdU加压筛选重组病毒3~5次。经过挑斑纯化、扩增培养后用PCR、IFA检测筛选表达的FMDVP1蛋白。用这2株病毒分别免疫BALB/c小鼠,通过SN和ELISA方法检测免疫小鼠的抗体水平。成功地获得了2株可以正确表达目的蛋白的重组伪狂犬病毒rPRVP1和rPRV3CP1。2株重组病毒均可诱导小鼠产生特异性抗FMDV抗体,两重组病毒各项免疫指标之间没有明显的差异。Two recombinant viruses expressing P1 gene and P1,3C genes of FMDV using PRV as vectors were constructed.The immunogenicity of two recombinant viruses were evaluated.The recombinant transfer plasmids pIESZP1 and pUTK3CP1 were infected into Vero cells which were infected 0.1MOI PRV.The virus was collected 120 hours after culture and then the virus was selected 3-5 cycles using blue plague and BrdU.The expression of P1 protein were identified by PCR and IFA after plague purification and expanded culture.BALB/c mice were immuned using the two recombinant viruses and the antibody level was examined by SN and ELISA.Two recombinant viruses rPRVP1 and rPRV3CP1 using PRV as vectors,which can correctly express P1 protein of FMD were obtained.Two recombinant viruses could induce the specific antibodies against expressed P1 protein of FMD.There was no distinctive immunity difference.

关 键 词：口蹄疫病毒 重组伪狂犬病毒 P1基因 实验免疫 

分 类 号：S852.65[农业科学—基础兽医学]