间接免疫荧光染色检测鸭源副黏病毒及在鸭体内的抗原定位  

Application of indirect immuno-fluorescent assay for detection and antigen location of duck paramyxovirus in paraffin sections

作　　者：吴焕荣[1] 刁有祥[1] 李宏梅[1] 颜赟[1] 孙杰[1] 李瑶瑶[1] 孙洪磊[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《中国兽医学报》2011年第2期189-193,共5页Chinese Journal of Veterinary Science

基　　金：山东省科技攻关项目(2007GG30009001)

摘　　要：以抗新城疫病毒F蛋白的单克隆抗体为一抗,建立了间接免疫荧光染色法(IFA)检测石蜡切片中鸭副黏病毒(DPMV)的方法。以建立的IFA对DPMV人工感染鸭的各组织器官进行检测,结果显示:各组织器官均能检测到DPMV,但不同时间取样,阳性信号分布的器官不同。脾脏、胸腺、法氏囊、肠道、肝脏、肺脏DPMV的阳性检出率较高,表明这些器官为DPMV的主要靶器官。在所检测的阳性组织中,病毒抗原分布在细胞浆中。IFA检测石蜡切片中的DPMV具有直观、特异性强的优点,是对DPMV进行检测和抗原定位的良好方法。An immuno-fluorescence assay by NDV monoclonal antibody was developed to detect antigen of duck paramyxovirus in paraffin sections.The IFA was applied to detect the DPMV antigen in different organs of the artificially infected ducks.The results showed that DPMV antigen can be detected in all the which were sampled tissues.Just only sampled at different time,the distribution of positive signals were in different tissues.The research suggest that the positive detection rate was high in spleen,thymus,bursa of Fabricius,intestine,liver and lung,which are the main target organs of DPMV.The antigens distributed in the cytoplasm of the infected cells.IFA should be a sensitive and specific method for detecting DPMV antigen in paraffin sections and could be used for diagnosis and antigen location of DPMV.

关 键 词：间接免疫荧光 鸭副黏病毒 检测 抗原定位 

分 类 号：S852.65[农业科学—基础兽医学]