黄芪多糖对SLT-Ⅱe诱导大鼠肠黏膜微血管内皮细胞分泌细胞因子的影响  

Effects of astragalus polysaccharin on the secretion of cytokine in rat intestinal microvascular endothelial cells induced by SLT-Ⅱe

作　　者：周宏超[1,2] 胡格[2] 范光丽[2] 刘钟杰[3] 穆祥[2] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]北京农学院兽医学(中医药)北京市重点实验室,北京昌平102206 [3]中国农业大学动物医学院,北京100094

出　　处：《中国兽医学报》2011年第2期222-226,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30671543);北京自然科学基金项目(6021001);北京市属市管高等学校人才强校计划项目(BAHED)

摘　　要：通过检测黄芪多糖对SLT-Ⅱe诱导的大鼠肠黏膜微血管内皮细胞分泌NO、ET-1、PGI2、TXA2、P-选凝素及sICAM-1的浓度变化,探索中药复方主要成分黄芪多糖对仔猪水肿病的疗效机制。将培养的肠黏膜微血管内皮细胞分为对照组、SLT-Ⅱe组、不同浓度黄芪多糖处理组等5个组,采用ELISA法测定了培养3、6、9和12 h时细胞培养上清液中NO、ET-1、PGI2、TXA2、P-选凝素及sICAM-1浓度的变化。10 mg/L黄芪多糖可下调SLT-Ⅱe诱导的大鼠肠黏膜微血管内皮细胞NO、ET-1、TXA2及sICAM-1的分泌,12 h内使NO、ET-1、TXA2及sICAM-1的分泌浓度接近正常水平;5、10、15 mg/L黄芪多糖不能下调SLT-Ⅱe诱导的大鼠肠黏膜微血管内皮细胞PGI2及P-选凝素的分泌。黄芪多糖通过抑制SLT-Ⅱe诱导肠黏膜微血管内皮细胞NO、ET-1 TXA2及sICAM-1的过量分泌,缓解肠道微循环障碍,减轻局部炎症反应。Determining the concentration of NO,ET-1,PGI2,TXA2,P-selectin and sICAM-1 in intestinal microvascular endothelial cells of rats induced by SLT-Ⅱe to study the functional mechanism of astragalus polysaccharin on edema disease of swine.The cultured RIMECs in vitro are divided into control group,SLT-Ⅱe group and three different treatment groups.Using ELISA to detect the concentration of NO,ET-1,PGI2,TXA2,P-selectin and sICAM-1 of cell supernatant for 3 h,6 h,9 h and 12 h culture,respectively.The results showed that 10 mg/L astragalus polysaccharin reduced the secretion of NO,ET-1,TXA2 and sICAM-1 remarkably.The concentration of NO,ET-1,TXA2 and sICAM-1 reached the normal level within 12 h.The secretion of PGI2 and Pselectin was reduced by adding of 5,10 and 15 mg/L astragalus polysaccharin.Astragalus polysaccharin could reduce the secretion of NO,ET-1,TXA2 and sICAM-1 in IMECs of rat,and alleviate the inflammation response.

关 键 词：SLT-Ⅱe 肠黏膜微血管内皮细胞 NO ET-1 PGI2 TXA2 P-选凝素 sICAM-1 黄芪多糖 

分 类 号：S853.76[农业科学—临床兽医学]