长效盐酸多西环素注射液在猪体内残留的消除规律  

Residual elimination of long-acting doxycycline hydrochloride injection in swines

作　　者：李媚[1] 刘伟坚[1] 黄显会[1] 曾振灵[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《中国兽医学报》2011年第2期227-231,236,共6页Chinese Journal of Veterinary Science

基　　金：国家"十一五"科技支撑重点项目(2006BAD31B06)

摘　　要：在常规饲养条件下,对35头健康成年猪按10 mg/kg体质量的剂量肌肉注射10%长效盐酸多西环素注射液,给药2次,给药间隔时间为48 h。第2次给药后12 h及2、5、9、14、192、5 d分别屠宰5头猪,分别采取每头猪的肌肉、肝脏、肾脏、皮肤+脂肪和注射位点肌肉等5种组织,用高效液相色谱法进行残留量测定。结果表明:在第2次给药后19 d,多西环素在各组织均能检测到,且残留均低于残留限量。多西环素残留浓度大小顺序:注射部位(肾脏(肝脏(皮脂(肌肉。采用WT1.4软件制定的统计方法来处理猪组织中药物浓度-时间数据,以制定休药期。In routine breeding conditions,35 healthy adult pigs were randomly divided into seven groups,5 pigs each group.The long-acting doxycycline hydrochloride injection was administered intramuscularly at a dose of 10 mg/kg.Five pigs were sacrificed with exsanguinations at 12 h,2,5,9,14,19 and 25 d after last administration,and samples of rump muscle,injection site,liver,kidney and abdominal fat were collected.The doxycycline concentrations in muscle,injection site,liver,kidney and skint were determined by HPLC method.Tissue residues of doxycycline was below the maximum residue limit（MRL） after 19 days.In all of the detected tissues,injection site had the highest concentration,and then followed by kidney,liver and fat,while muscle had the lowest concentration.The concentration-time data of doxycycline were analyzed by WT1.4 software to get the WDT.

关 键 词：多西环素 猪组织 残留 休药期 高效液相色谱 

分 类 号：S859.7[农业科学—临床兽医学]