筛选甘蔗SPSⅢ启动子诱饵片段结合蛋白的酵母单杂交诱饵质粒的构建  

Construction of Yeast One-hybrid Bait Plasmids for Screening the Binding Proteins of Sucrose SPSⅢ Promoter Cis-elementonstruction

作　　者：邹丽娟[1,2,3] 高玉娜[1,2,3] 周平[4] 叶冰莹[1,2,3] 陈由强[1,2,3] 陈如凯[2] 

机构地区：[1]福建师范大学生命科学学院,福建福州350108 [2]农业部甘蔗遗传改良重点开放实验室,福建福州350108 [3]福建省发育与神经生物学重点实验室,福建福州350108 [4]福建省农业科学院果树研究所,福建福州350013

出　　处：《福建师范大学学报（自然科学版）》2011年第1期76-79,共4页Journal of Fujian Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(30871575);现代农业产业技术体系建设专项资金(nycytx-024-01-20);福建省科技厅资助项目(2007F5034)

摘　　要：主要构建了可以与酵母染色体发生重组的质粒pAbAI-Bait,并转化大肠杆菌DH5α感受态细胞获得转化子,将阳性克隆进行PCR与DNA测序的方法鉴定,结果表明:酵母单杂交中报告质粒pAbAI-Bait构建成功,可用于酵母单杂交体系.It has constructed the pAbAI-Bait plasmid which could be integrated into the yeast genome.Then transformed into E.coli DH5α,and the transformant were obtained.The recombinant were confirmed by PCR and DNA sequencing.The result indicated that the plasmid pAbAI-Bait has been successfully constructed and can be used in yeast hybrid system.

关 键 词：酵母单杂交 蔗糖磷酸合成酶基因Ⅲ 转录因子 

分 类 号：Q946[生物学—植物学]