抗CD95单克隆抗体诱导的活化T细胞凋亡时转录因子nur77的表达  

TRANSCRIPTION FACTOR NUR77 EXPRESSION IN APOPTO TIC T CELLS INDUCED BY ANTI CD95 MONOCLONAL ANTIBODY

作　　者：成军[1,2] 斯崇文[1,2] 王勤环[1,2] 刘妍[1,2] 洪卫国[1,2] 钟彦伟[1,2] 杨继珍 

机构地区：[1]解放军302医院传染病研究所基因治疗研究中心 [2]北京医科大学第一医院传染病教研室

出　　处：《免疫学杂志》1999年第3期177-179,共3页Immunological Journal

摘　　要：以抗ＣＤ９５的特异性单克隆抗体（ＣＨ－１１）诱导人Ｔ细胞Ｊｕｒｋａｔ细胞系发生凋亡，Ｊｕｒｋａｔ细胞在发生凋亡过程中出现典型的ＤＮＡ梯形片段化，以荧光染料（ＰＩ）标记的流式细胞学技术对于发生凋亡的细胞进行定量测定，证实发生凋亡的细胞百分比在各个时间点上为６．１％～４３．５％之间。以电泳泳动迁移率（ＥＭＳＡ）对于发生凋亡的细胞核中转录因子ｎｕｒ７７的表达进行了研究，发现以抗ＣＤ９５单克隆抗体诱导的活化Ｔ细胞发生凋亡时，ｎｕｒ７７转录因子表达水平有显著提高。研究结果对了解细胞凋亡时的信号转导与分子生物学机制具有重要意义。The apoptotic Jurkat cells induced by anti CD95 monoclonal antibody CH 11 were confirmed by DNA fragmentation and flow cytometry assay with PI staining.6.1% to 43.5% of the cells were dying via apoptosis after induction with 10μg/L of anti CD95 monoclonal antibody CH 11 for 0～24 hours.The transcription factor expression in apoptotic cells was studied by the electrophoretic mobility shift assay(EMSA),indicating that nur77 expression maybe involved in apoptosis regulating process of activated T cells induced by anti CD95 antibody.

关 键 词：CD95 细胞凋亡 T细胞 转录因子 单克隆抗体 

分 类 号：Q513.2[生物学—生物化学] Q78