流感病毒核酸检测方法研究进展  

Progress on Nucleic Acid Detection Technology of Influenza Virus

作　　者：徐敏[1] 向华[1] 

机构地区：[1]广东省农业科学院兽医研究所广东省兽医公共卫生开放实验室,广东广州510640

出　　处：《动物医学进展》2011年第1期89-92,共4页Progress In Veterinary Medicine

基　　金：广东省科技厅科技减灾恢复生产应急专项项目(2009B020600001);广东省农业科学院院长基金项目(20090203)

摘　　要：流感病毒可以引起人和动物高度接触性传染性疾病,对公共卫生和畜禽养殖业均造成极大的威胁。因此,准确而又快速的诊断方法对流感的防控起着非常重要的作用。近年来,流感病毒的核酸诊断技术发展较快,主要有RT-PCR、实时荧光定量RT-PCR、基因芯片、逆转录环介导等温扩增(RT-LAMP)等。论文对这些诊断技术的基本原理和应用现状进行了综述,并对其发展前景进行了展望。Because of causing serious contagious infectious diseases of human and animals,influenza virus have caused a great threat to public health and animal husbandry.Therefore,accurate and rapid diagnostic technology plays a very important role in influenza prevention and control.In recent years,influenza virus detection techniques developed rapidly,mainly including RT-PCR,real-time quantitative RT-PCR,gene chip,reverse transcription loop-mediated isothermal amplification（RT-LAMP） and so on.In this paper,applications and basic principles of these detection techniques were reviewed,and their development was prospected.

关 键 词：流感病毒 逆转录-聚合酶链反应 实时荧光定量RT-PCR 基因芯片 环介导等温扩增 

分 类 号：S854.43[农业科学—临床兽医学]