人肝肾微粒体1抗原的提取及其抗体的检测  

Extraction of LKM 1 and screening of anti LKM 1 antibody in patients with ALT increase

作　　者：王达[1] 仲人前[1] 于嘉屏[1] 孔宪涛[1] 

机构地区：[1]第二军医大学附属长征医院实验诊断科,上海200003

出　　处：《中华医学检验杂志》1999年第4期222-224,共3页

摘　　要：目的　进一步了解自身免疫性肝炎的致病机理。方法　测定肝功能异常患者的肝肾微粒体（ Ｌ Ｋ Ｍ）１ 的自身抗体阳性情况。在重组质粒表达的基础上，用亲和柱层析进一步纯化，采用免疫印迹法检测抗 Ｌ Ｋ Ｍ１ 抗体。结果　在６０５ 例丙氨酸氨基转移酶（ Ａ Ｌ Ｔ） 升高的成人患者组内，检出 Ｌ Ｋ Ｍ１ 自身抗体阳性２４ 例，阳性率为３９７ ％ ，５０ 例 Ａ Ｌ Ｔ升高的小儿患者中检出阳性６ 例，占１２ ％ 。两组比较，差异有非常显著意义（ Ｐ＜ ００１） 。而５０ 名正常人及４０ 例丙型肝炎病毒（ Ｈ Ｃ Ｖ） Ｒ Ｎ Ａ 阳性的丙型肝炎患者中，无一发现 Ｌ Ｋ Ｍ１ 自身抗体阳性。结论　 Ｌ Ｋ Ｍ１ 自身抗体可作为自身免疫性肝炎（ Ａ Ｉ Ｈ）的一项重要检测指标。Objective To extract human recombinant liver kidney microsomal 1 antigen(LKM 1) and screen LKM 1 autoantibody in patients with ALT increase.Methods After expressing LKM 1 in prokaryote by constructing recombinant expressive plasmid successfully, the fusion protein was purified by affinity chromatography. Anti LKM 1 antibody was tested in patients with ALT increase by immunobloting.Results LKM 1 autoantibody was found in 3.97%(24/605) of adults with ALT increase, and 12.00%(6/50) of children with ALT increase. The positive percentage of LKM 1 autoantibody was significantly different ( P <0.01) between the two groups. On the contrary, neither 40 patients with HCV infection nor 50 healthy controls were positive for LKM 1Ab. Conclusion It is useful to detect LKM 1 autoantibody for the diagnosis of AIH.

关 键 词：微粒体 自身抗体 免疫印迹法 肝炎 LKM-1 

分 类 号：R512.604[医药卫生—内科学] R446.62[医药卫生—临床医学]