外源性一氧化碳释放分子对脓毒症小鼠血小板膜糖蛋白CD41、CD61和CD62p表达的影响  

Effect of extrinsic carbon monoxide-releasing molecules Ⅱ on expressions of CD41/CD61 and CD62p on the platelet membrane surface in mice with CLP

作　　者：杨国涛[1] 孙炳伟[1] 邹向前[1] 王敏[1] 

机构地区：[1]江苏大学附属医院烧伤整形科,江苏镇江212001

出　　处：《江苏大学学报（医学版）》2011年第1期1-4,共4页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(30772256;81071546);江苏省自然科学基金资助项目(BK2008237);江苏省政府留学基金资助项目(2008-K002)

摘　　要：目的:观察外源性一氧化碳释放分子(carbon monoxide releasing-moleculesⅡ,CORM-2)对盲肠结扎和穿孔(cecal ligation and puncture,CLP)脓毒症模型小鼠血小板膜糖蛋白CD41、CD61和CD62p表达的影响。方法:C57BL/6小鼠60只,随机均分成4个组,假手术组、CLP组、CLP+CORM-2组和高浓度氧预处理+CLP+CORM-2组,每组各15只,每组再分6 h、12 h和24 h 3个时间段。用流式细胞仪测定血小板膜上CD41、CD61的平均荧光强度及CD62p的阳性率。结果:各时间段高氧预处理联合CORM-2干预组血小板膜糖蛋白CD61和CD62p的表达较CLP组明显下降,接近于假手术组水平。结论:CORM-2可降低CLP小鼠血小板膜糖蛋白CD61和CD62p的表达,抑制血小板的活化。Objective： To investigate the effects of extrinsic carbon monoxide-releasing moleculesⅡ（CORM-2） on the expressions of CD41/CD61 and CD62p on the platelet membrane surface in mice with cecal ligation and puncture（CLP）.Methods： Sixty male C57BL/6 mice were randomly divided into sham,CLP,CLP＋CORM-2,O2＋CLP＋CORM-2 groups.One group were randomly divided into 6 h,12 h and 24 h.The mean immunofluorescence intensity of CD41,CD61or positive percentage of CD62p was measured by the flow cytometry（FCM）.Results： The mean fluorescence intensity of CD61 and positive percentage of CD62p on platelet membrane respectively in O2＋CORM-2＋CLP group were significantly decreased while compared with CLP group and were approximated with control Sham group.Conclusion： The extrinsic carbon monoxide-releasing moleculesⅡ may induce down regulation of platelet membrane glycoprotein CD61 and CD62p expression in CLP mice,with inhibiting that platelet activation.

关 键 词：外源性一氧化碳释放分子 盲肠结扎和穿孔小鼠 血小板膜糖蛋白 

分 类 号：R631.2[医药卫生—外科学]