肝硬化大鼠肝组织中组织胺H_2受体基因的mRNA表达  

mRNA expression of histamine H 2 receptor in hepatic tissues of cirrhotic rats

作　　者：陈雷[1] 胡继舟[1] 冷希圣[1] 杜如昱[1] 

机构地区：[1]北京医科大学人民医院外科

出　　处：《中华实验外科杂志》1999年第4期323-324,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金

摘　　要：目的探讨肝硬化大鼠肝组织中组织胺Ｈ２受体基因的ｍＲＮＡ表达情况。方法逆转录聚合酶链反应方法从Ｗｉｓｔａｒ大鼠脑组织中合成Ｈ２受体的ｃＤＮＡ片段，克隆入载体中测序。用同位素标记ｃＤＮＡ片段后用于Ｎｏｒｔｈｅｒｎ印迹杂交。采用Ｎｏｒｔｈｅｒｎ印迹杂交法检测了１０例肝硬化Ｗｉｓｔａｒ大鼠及１０例正常对照组大鼠肝组织的Ｈ２受体ｍＲＮＡ。结果正常及肝硬化大鼠肝组织中均有Ｈ２受体的ｍＲＮＡ表达，且肝硬化组的表达量显著低于正常对照组。结论肝硬化时肝组织Ｈ２受体基因的转录减少是造成肝硬化时Ｈ２受体蛋白减少的重要原因，在门静脉高压症的形成和维持中起重要作用。Objective\ To investigate mRNA expression of histamine H 2 receptor (H 2R) in hepatic tissues of cirrhotic rats with portal hypertension. Methods\ Reverse transcription and polymerase chain reaction was used to synthesize cDNA of H 2R gene from Wistar rats' brain tissues and then cDNA was cloned into vector to be sequenced. The H 2R cDNA was labeled with isotope α 32 P and used as special probe in Northern blot hybridization. Then, mRNA expression of H 2R in hepatic tissues of 10 cirrhotic rats with portal hypertension and 10 normal control rats was detected by using Northern blot hybridization. Results\ mRNA expression of H 2R in hepatic tissues of both normal and cirrhotic rats was found, but in cirrhotic rats was significantly lower than that of normal rats ( P <0.01).Conclusion\ The decrease of H 2R gene transcription is an important cause of the decrease of H 2R proteins in the hepatic tissues of cirrhotic rats with portal hypertension, which might play an important role in the pathogenesis and maintenance of portal hypertension.

关 键 词：门脉高压症 组织胺H2受体 MRNA 基因表达 

分 类 号：R657.340.2[医药卫生—外科学]