噬菌体生物扩增法检测结核分枝杆菌氧氟沙星耐药性研究  

Detection of ofloxacin resistance in M.tuberculosisby phage-amplified biologically assay

作　　者：王庆[1] 徐东芳[1] 

机构地区：[1]安徽省结核病防治研究所,合肥230022

出　　处：《中国防痨杂志》2011年第1期43-46,共4页Chinese Journal of Antituberculosis

基　　金：安徽省卫生厅医学科研资助基金项目(2008B080)

摘　　要：目的建立快速测定结核分枝杆菌氧氟沙星耐药性的噬菌体生物扩增法,并探讨其在结核分枝杆菌氧氟沙星耐药性测定中的应用价值。探讨噬菌体检测技术(PhaB)快速检测氧氟沙星抗结核药物耐药性的可能性,分析其临床应用价值。方法应用PhaB测定205株结核分枝杆菌(MTB)临床分离株对氧氟沙星的耐药性,并与绝对浓度法药敏结果比较,对两法检测结果不一致的菌株进行比例法测定。结果以绝对浓度法药敏结果为判断标准,则PhaB检测OFX的敏感度、特异度、阳性和阴性预测值(PPV、NPV)及准确度分别为95.4%、93.2%、91.2%、96.5%和94.1%,共有12株菌株PhaB法药敏结果与绝对浓度法检测结果不符,其中9株PhaB法药敏结果与比例法相符合。结论 PhaB检测氧氟沙星药敏结果与绝对浓度法有较高的一致率,可作为MTB耐药性的快速筛选方法。Objective To develop a rapid method to detect Mycobacterium tuberculosis（MTB） resistance to ofloxacin by phage-amplified biologically（PhaB） assay,and to evaluate its clinical value.Methods Compared with absolute concentration method,205 MTB clinical isolates were detected their resistance to ofloxacin.If the results from these two methods are inconsistent,the ofloxacin susceptibility of this strain would be was determined by the proportion method.Results As the results from the absolute concentration method was considered as the golden standard,the sensitivity,specificity,positive and negative predictive value（PPV and NPV） as well as accuracy of PhaB assay for the ofloxacin susceptibility was 95.4%,93.2%,91.2%,96.5% and 94.1%,respectively.The results from 12 isolates were different between PhaB assay and the absolute concentration method,in which the results from PhaB assay in 9 isolates were identical with that from the proportion method.Conclusions The results of ofloxacin susceptibility from PhaB assay had higher concordance with that from the absolute concentration method.PhaB assay can be used for rapid screening of drug susceptibility in MTB.

关 键 词：分枝杆菌 结核 微生物敏感性试验 表型 氧氟沙星 

分 类 号：R446.5[医药卫生—诊断学]