抗生素所致腹泻大鼠肠屏障功能损伤及乳酸杆菌保护机制的研究  

Protective effect of Lactobacillus rhamnosus GG on the damage of gut barrier function in SD rats with antibiotics induced diarrhea

作　　者：张琳[1] 乔木 梁庆红[1] 陈晓晴[1] 黄丽华[1] 李春香[3] 

机构地区：[1]河北医科大学第三医院儿科,河北石家庄050051 [2]秦皇岛市人民医院,河北秦皇岛066001 [3]河北医科大学中医学院,河北石家庄050091

出　　处：《中国微生态学杂志》2011年第1期38-43,48,共7页Chinese Journal of Microecology

摘　　要：目的探讨抗生素对所致腹泻大鼠肠道屏障功能、肠道菌群结构和肠道细菌移位的影响及乳酸杆菌制剂的保护机制。方法采用细菌培养法动态测定抗生素所致腹泻大鼠肠道菌群变化及肠系膜淋巴结、肝脏、脾脏和结肠组织的移位细菌量;应用光镜和电子显微镜观察肠黏膜组织超微结构变化。结果应用抗生素可致大鼠腹泻,肠道菌群失调,肠黏膜组织受损,发生肠道细菌移位。大肠埃希菌攻击可加重肠道菌群失调和肠黏膜损伤程度,促发细菌移位发生。乳酸杆菌可扶正肠菌群结构,修复损伤的肠黏膜,抑制肠细菌移位发生。结论阐明了抗生素、肠黏膜屏障功能、肠道菌群结构和肠道细菌移位间的互为因果,相互影响的关系。微生态制剂在维持机体微生态平衡、修复肠黏膜方面具有保护作用。Objective To study the interrelation of gut mucosa barrier function,intestinal microflora and bacteria translocation and the protective mechanisms of Lactobacillus rhamnosus GG（LGG） on the damage of gut barrier function in SD rats with antibiotics induced diarrhea.Method The dynamic changes and bacterial translocation phenomena of intestinal flora in SD rats were observed by bacterial culture after using antibiotics,and bowel tissue changes were observed with electronmicroscopic method.Result Imbalance of intestinal flora was observed in rats after application of antibiotics;bacterial translocations occurred,and the degree of damage to gut barrier function were varied;Escherichia coli pathogenic attack had increased the degree of bacterial translocation and the damage to gut barrier function.Lactobacillus intervention group displayed the effect of reducing the degree of bacterial translocation and lessening the damage to gut barrier function.Conclusion The correlations,after using antibiotics,among intestinal flora,bacterial translocation phenomenon and gut mucosa barrier function have been clarified.Early intervention of microecological agents may provide a protection for gut barrier function.

关 键 词：抗生素 肠黏膜屏障 肠道菌群结构 细菌移位现象 乳酸杆菌 

分 类 号：R378[医药卫生—病原生物学]