机械生长因子对骨骼肌卫星细胞激活的影响及与其增殖的量效关系  

Effects of Mechano Growth Factor on Activation and the Dose-dependent Proliferation of Skeletal Muscle Satellite Cells in Vitro

作　　者：吴嵽[1] 陆耀飞[1] 

机构地区：[1]上海体育学院运动科学学院,上海200438

出　　处：《体育科学》2011年第2期64-69,共6页China Sport Science

基　　金：上海市教育委员会科研项目(05IZ02);上海市第三期重点学科资助项目(S30802)

摘　　要：目的:通过离体实验,观察机械生长因子(MGF)对于骨骼肌卫星细胞(SC)的激活作用及促进其增殖的量效关系,探讨MGF对SC在细胞水平的调节机制,为骨骼肌损伤的修复提供理论依据。方法:原代SC取自4周龄雄性SD大鼠双侧的腓肠肌与比目鱼肌。使用不同浓度的MGF干预第三代细胞后测定增殖效果。血清饥饿后通过MGF和DMEM干预骨骼肌卫星细胞,使用流式细胞仪检测其所处细胞周期,判断激活情况。结果:干预48h后,25ng/ml组、50ng/ml组A值显著高于对照组(P<0.01),100ng/ml组、200ng/ml组与对照组之间差异没有显著性(P>0.05);96h后25ng/ml组、50ng/ml组、100ng/ml组、200ng/ml组之间差异没有显著性(P>0.05)。25ng/mlMGF干预G0期的SC24h后MGF组低于对照组(P<0.01)。结论:MGF可以促进SC增殖;MGF可以激活4周龄SD大鼠的G期SC。Objective：This paper observed the effects of MGF on activation and the dose-dependent manner on proliferation of SC in vitro which increasing the number available for local repair,as well as the mechanism at the levels of molecular.Method：The primary passage SC was derived from gastrocnemius muscle and soleus muscle of the Sprague-Dawley rats（4 weeks of age,male）.The third passage SC was treated with MGF and the cell proliferation rate was measured by cell proliferation assay.The third passage SC was serum starved and treated with MGF and DMEM.The cell cycle was detected with Flow Cytometry.Result：After 48h,values of the operated groups which treated with MGF of 25ng/ml and 50ng/ml were significantly higher than that of normal control group（P0.01）.There was no significant difference between 100ng/ml group and 200ng/ml group（P0.05）.After 96h,there was no significant difference among the operated groups of 25ng/ml,50ng/ml,100ng/ml and 200ng/ml（P0.05）.There were significant differences between the operated group and the normal control group,the proportion of the SC in G0/G1 phase of operated group treated with 25ng/ml MGF was significantly lower than that in normal control group after 24h（P0.01）.Conclusion：MGF increases skeletal muscle SC proliferation.MGF appears to initiate skeletal muscle SC activation of 4-week-old Sprague-Dawley rats.

关 键 词：骨骼肌卫星细胞 机械生长因子 细胞培养 细胞增殖 细胞激活 鼠 动物实验 

分 类 号：G804.2[文化科学—运动人体科学]