BED捕获酶免疫试验方法检测HIV-1新发感染的系统评价  

A system review on the application of BED-capture enzyme immunoassay in detecting new HIV-1 infection

作　　者：陶钧[1] 赵晶[1] 刘勇[2] 孟令章[1] 于石成[3] 蒋岩[1] 肖瑶[1] 

机构地区：[1]中国疾病预防控制中心性病艾滋病中心参比室,北京102206 [2]贵州省疾病预防控制中心艾滋病防治所 [3]中国疾病预防控制中心卫生统计研究室

出　　处：《中华流行病学杂志》2011年第2期177-181,共5页Chinese Journal of Epidemiology

摘　　要：目的 分析BED捕获酶免疫试验（BED-CEIA）是否适合在大范围人群内应用,对BED-CEIA与队列研究获取HIV-1新发感染率的一致性和BED-CEIA错分现象对新发感染率结果影响进行系统评价.方法 利用系统评价的方法分析符合纳入标准的文献,其中10篇入选BED-CEIA与队列研究获取HIV-1新发感染率一致性的探讨,11篇文献入选BED-CEIA的错分现象研究.结果 BED-CEIA计算的HIV-1新发感染率与队列观察的新发感染率一致性与研究地区、研究设计相关,非洲地区的一致性较差,BED-CEIA的样本采集时间与队列随访同步研究获取的新发感染率一致性较好.BED-CEIA的错分现象研究共收集到7303份长期感染者（LTI）样本,错分为新近感染的样本数为432,错分粗率为5.9%,其95%CI：5.36～6.44.BED-CEIA的错分率与HIV-1感染时间和CD4＋T淋巴细胞计数相关,而与人群（中国女性性工作者、静脉吸毒人群）和地区（中国与非洲地区）无显著相关.结论 BED-CEIA计算的HIV-1新发感染率与队列研究观察的新发感染率,其一致性随研究地区和研究设计的不同而有差异;BED-CEIA错分现象与HIV-1感染者的免疫状况相关.Objective To study whether BED-capture enzyme immunoassay （CEIA） is feasible to be used in wide-ranging population, we collect papers and conference abstracts related to BED-CEIA and HIV-1 incidence. Methods 10 papers are included for the discussion, regarding the concordance between the estimated HIV-1 incidence from BED-CEIA and the results from a cohort studies; and 11 papers are selected to discuss the related misclassification on the estimation of HIV-1incidence. Results Concordance between the two sets is related to the districts and design of research. Results from Africa are not so satisfactory, but researches, those BED-CEIA samples are collected during the follow-up of the cohort study, have shown better outcomes than other ones. There are totally 7303 samples of LTI （long-term infections） collected for analyzing the misclassification of BED-CEIA. 432 LTI are misclassified as new infections, making the raw rate of misclassification as 5.9% with 95% confidential interval between 5.36 and 6.44. Data from systematic review shows that the BED-CEIA＇s misclassification rate relates to the count of CD4＋T lymphocytes and time after the infection but has no relation to the classification of sub-populations （female sexual workers and intravenous drug users in China） and districts （China and Africa）. Conclusion Our results reveal that the misclassification is relevant to the immune-status of the infected persons.

关 键 词：BED捕获酶免疫试验 HIV-1感染率 系统评价 

分 类 号：R512.91[医药卫生—内科学]