纳米粒子标记激光诱导荧光免疫分析测定癌胚抗原  

Immunoassay for Carcinoembryonic Antigen Based on Fluorescence Induced by Laser and Labeled with Nanoparticles

作　　者：张晓明[1] 章竹君[1] 薛盼[1] 

机构地区：[1]f陕西师范大学化学与材料科学学院陕西省生命分析重点实验室,西安710062

出　　处：《化学学报》2011年第2期199-203,共5页Acta Chimica Sinica

摘　　要：建立了一种核壳型SiO2荧光纳米粒子标记的激光诱导荧光免疫测定的新方法.该方法用Ru(bpy)32+掺杂的核壳型SiO2荧光纳米粒子为标记物,采用双抗体夹心法,在载玻片上固定鼠抗人癌胚抗原(CEA)单克隆抗体,加入1～2μL样品,再加入纳米粒子标记的另一种鼠抗人癌胚抗原(CEA)单克隆抗体.识别后在光导纤维激光诱导荧光毫米阵列检测平台上测量荧光信号,进行定量.在优化条件下,荧光强度和癌胚抗原的量在1～80 pg的范围内具有良好的线性关系,相关系数r=0.9933,方法的检出限为0.3 pg(20 amol).对40 pg的CEA平行测定5次,相对标准偏差为5.5%.A new immunoassay method for Carcinoembryonic Antigen based on fluorescence induced by laser and labeled with nanoparticles is presented.Core-shell SiO2 fluorescent nanoparticles were used as la-bels,＂sandwich＂ model was used.Mouse anti-carcinoembryonic antigen（CEA） monoclonal antibody was immobilized on slides,then 1～2 μL CEA was added,and the other mouse anti-CEA monoclonal antibody labeled with nanoparticles was added,after CEA and antibody recognized,the fluorescent signal was quanti-fied with an laser-induced fluorescence millimeter array detection platform based on optical fiber.The fluo-rescence intensity has a linear relationship with the concentration of CEA in the range of 1～80 pg,with the absolute limit detection of CEA being 0.3 pg.The relative standard deviation（RSD） for 5 parallel measure-ments of CEA（40 pg） was 5.5%.

关 键 词：纳米粒子 激光诱导荧光 阵列传感器 癌胚抗原 

分 类 号：TB383.1[一般工业技术—材料科学与工程]