复方板蓝根颗粒抗病毒成分HPLC-UV指纹图谱及LC-ESI-MS^n研究  

Studies on Fingerprint Chromatograms of Anti-virus Component of Compound Indigowoad Root Granule by HPLC-UV and LC-ESI-MS^n

作　　者：闫峻[1] 刘舒[2] 皮子凤[2] 宋凤瑞[2] 刘忠英[1] 刘志强[2] 

机构地区：[1]吉林大学药学院,长春130021 [2]中国科学院长春应用化学研究所,长春130022

出　　处：《化学学报》2011年第2期204-208,共5页Acta Chimica Sinica

基　　金：国家"十一五"科技支撑计划(No.2006BAI08B03-02)资助项目

摘　　要：建立了复方板蓝根颗粒提取物高效液相色谱(HPLC-UV)指纹图谱分析方法.确定了10批不同来源复方板蓝根颗粒提取物的19个共有峰.各提取物的HPLC-UV指纹图谱与对照指纹图谱比较,相似度均在93%以上.利用液相色谱与质谱联用(LC-ESI-MSn)技术对主要共有峰的结构进行了鉴定.药效学研究表明,复方板蓝根颗粒提取物有抗病毒活性.该指纹图谱精密度、稳定性和重现性良好,可作为复方板蓝根颗粒的质量评价方法.The fingerprint chromatograms of the extracts from Compound Indigowoad Root Granule was established by HPLC-UV.Nineteen common peaks were found in fingerprint chromatograms of the extracts from Compound Indigowoad Root Granule and good similarities with correlation coefficients over 93% were found between the fingerprint chromatograms of the extracts from different sources.The main common peaks were identificated by HPLC-MSn.Pharmacodynamic studies have shown that the extract from Com-pound Indigowoad Root Granule had anti-viral activity.This method with desirable accuracy,stability and repeatability can be used for the quality evaluation of Compound Indigowoad Root Granule.

关 键 词：复方板蓝根颗粒 指纹图谱 液相色谱与质谱联用 抗病毒活性 

分 类 号：R286[医药卫生—中药学]