单个肝癌细胞的拉曼成像研究  

Mono-Hepatocellular Carcinoma by Raman Mapping

作　　者：孟令晶[1,2] 纪晓露[1] 李自达[1,2] 刘军贤[1] 李蕾[3] 姚辉璐 

机构地区：[1]广西师范大学物理科学与技术学院,广西桂林541004 [2]广西科学院生物物理实验室,广西南宁530003 [3]广西医科大学研究生院,广西南宁530021

出　　处：《激光与光电子学进展》2011年第2期52-56,共5页Laser & Optoelectronics Progress

基　　金：国家自然科学基金(60868002;30760278);广西科技攻关(桂科合10100019-22)资助课题

摘　　要：应用拉曼成像技术检测单个人体肝癌细胞,获得了单个肝癌细胞微区的拉曼光谱图谱,同时计算出786,1450和1658cm-1等特征峰的峰面积,这些特征峰分别归属于DNA,脂类和蛋白质,根据归一化后的数值在相应的细胞扫描位置成像,重构出这些物质的拉曼特征峰在肝癌细胞中的分布图。结果表明,应用这种方法可以很明确地看到DNA,脂类及蛋白质特征峰在细胞中的分布情况,通过荧光染色验证了成像系统的可靠性。因此,可以通过特征峰的成像图确定物质在细胞中的微区分布情况,为拉曼方法检测和诊断肝癌提供了可靠的依据。Raman mapping technique is applied to detect mono-hepatocellular carcinoma.The area of characteristic peak at 786,1450 and 1658 cm-1,which assigns to DNA,lipid and protein,is calculated.According to normalized values image in the corresponding scan location of the cells,the distribution of Raman characteristic peak of these materials in liver cell is reconstructed.The results show that,the distribution of DNA,lipid and protein is exhibited clearly by this Raman mapping system,and the reliability is validated by fluorescence staining.The micro distribution of material in the cells can be confirmed by the imaging of characteristic peak,which provides reliable evidence for Raman detection and liver cancer diagnosis.

关 键 词：光谱学 肝癌 拉曼 成像 图像 

分 类 号：O433.54[机械工程—光学工程]