HPLC法测定金嗓清音丸中丹皮酚的含量  

Content Determination of Paeonol in Jinsang Qingyin Pills by HPLC

作　　者：孔永红 康红钰[2] 

机构地区：[1]河南省驻马店市中心医院药剂科,驻马店市463000 [2]河南省漯河医学高等专科学校,漯河市462002

出　　处：《中国药房》2011年第8期749-750,共2页China Pharmacy

摘　　要：目的:建立金嗓清音丸中丹皮酚的含量测定方法。方法:采用高效液相色谱法测定该药中丹皮酚的含量。色谱柱为Agi-lent1100C18柱,流动相为甲醇-0.1%乙酸溶液(60∶40),流速为1.0mL·min-1,检测波长为274nm。结果:丹皮酚在0.688～22.000μg·mL-1(r=0.9998)浓度范围内与峰面积积分值线性关系良好;平均回收率为95.91%,RSD=2.81%。结论:该方法准确、可靠、专属性强,可用于金嗓清音丸的质量控制。OBJECTIVE： To establish a method for the content determination of paeonol in Jinsang qingyin pills. METHODS： The content of paeonol in Jinsang qingyin pills was determined by HPLC. The chromatographic conditions were Agilent 1100 C18 as column, methanol-0.1% acetic acid （60：40） as mobile phase at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 274 nm. RESULTS： The linear range of paeonol was 0.688~22.000 μg·mL-1 （r=0.999 8） with an average recovery of 95.91% （RSD = 2.81% ）. CONCLUSION： The method is accurate, reliable and specific for the quality control of Jinsang qingyin pills.

关 键 词：金嗓清音丸 丹皮酚 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]