丝裂霉素C对含重复序列质粒的大肠杆菌致死率的模型化  

Modeling on lethality rate of Escherichia coli containing plasmid with repeat sequence by mitomycin C

作　　者：赵宏宇[1] 蔡禄[1] 赵秀娟[1] 王晶妍[1] 

机构地区：[1]内蒙古科技大学生物工程与技术研究所,内蒙古包头014010

出　　处：《华西药学杂志》2011年第1期9-10,共2页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金(批准号:61072129;30460038);内蒙古自然科学基金重点项目(200508010102)

摘　　要：目的探索丝裂霉素C对含重复序列质粒的大肠杆菌致死作用的规律。方法采用均匀设计试验方法设计丝裂霉素C的处理时间和药物浓度,统计存活菌数,计算菌体致死率。结果得到了丝裂霉素C的浓度和作用时间与菌体致死率间的线性回归方程,建立丝裂霉素C作用的响应面。结论丝裂霉素C的浓度和作用时间与菌体致死率呈线性正相关。OBJECTIVE To explore the lethality law of E.coli containing plasmid pLC（GAA）42 treated by Mitomycin C.METHODS U＊6（64） uniform experiment method was used to investigate the acting time and concentration of Mitomycin C and bacteria count was obtained by bacterial colony counter,then the lethality rate were counted.RESULTS The regression equation between lethality rate and concentration,and time of Mitomycin C was obtained and the correlation coefficient was 0.907.On the basis of the regression equation,response surface of mitomycin C treating E.coli DH5α was established.CONCLUSION There exists positive correlation between concentration,acting time of Mitomycin C and lethality rate of E.coli containing plasmid pLC（GAA）42.

关 键 词：丝裂霉素C 均匀设计 大肠杆菌 致死率 重复序列 

分 类 号：R96[医药卫生—药理学] R979[医药卫生—药学]