顺铂联合热疗对卵巢癌铂敏感细胞株COC1和铂耐药细胞株COC1/DDP的影响  

Effects of Cisplatin and hyperthermia on apoptosis and drug resistance of ovarian cancer cell line COC1,COC1/DDP

作　　者：李玉玲[1] 尹如铁[1] 谢聪[1] 肖萍[1] 游小林[1] 

机构地区：[1]四川大学华西第二医院,四川成都610041

出　　处：《华西药学杂志》2011年第1期24-26,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的探讨热疗联合化疗对卵巢癌铂敏感细胞株COC1和铂耐药细胞株COC1/DDP增殖、凋亡的作用、逆转耐药细胞的耐药性及其可能机制。方法采用MTT法检测热疗及联合不同浓度的顺铂(1.25、2.5、5、10、20μg.mL-1)对卵巢癌细胞株COC1,COC1/DDP生长的抑制作用;RT-PCR法检测两种细胞株中Survivin基因及ERCC1基因mRNA表达的差异。结果细胞株COC1/DDP在42℃联合顺铂作用下凋亡率增加明显,细胞半数致死量由4.98μg.mL-1(37℃)降至1.82μg.mL-1(42℃),对顺铂的敏感性增加了2.78倍(P<0.01),COC1半数致死量无明显差异(P>0.05);42℃时细胞株中ERCC1基因和survivin基因的mRNA表达量均低于37℃时的表达(P<0.05)。结论热疗本身具有杀灭肿瘤细胞的作用,联合化疗可增强耐药株对顺铂的敏感性,一定程度上逆转了铂耐药性,其机制可能与降低survivin基因和ERCC1基因mRNA的表达有关。OBJECTIVE To investigate effect of hyperthermia,hyperthermia combined with chemotherapy on proliferation,apoptosis and platinum resistance of ovarian cancer cell lines COC1,COC1/DDP and try to explore their possible mechanisms.METHODS MTT method was performed to test growth inhibition ratios of hyperthermia and of cisplatin（1.25,2.5,5,10,20 μg·mL-1） at certain temperature of hyperthermia on cell COC1,COC1/DDP.RT-PCR was adopted to detect expression level of Survivin mRNA and ERCC1 mRNA of the two cell lines.RESULTS Apoptosis rate of COC1/DDP increased significantly when treated with cisplatin at the temperature of 42 ℃.The cell median lethal dose decreased from 4.98 μg·mL-1（37 ℃）to 1.82 μg·mL-1（42 ℃）,which was decreased by 2.78-fold compared with the former（P0.01）.Howerver,the median lethal dose of COC1 did not significantly changed although its apoptosis rate increased（P0.05）.Both expression level of survivin mRNA and ERCC1 mRNA of cell lines at 42 ℃ were significantly lower than that at 37 ℃（P0.05）.CONCLUSION Hyperthermia itself might kill tumor cells.Hyperthermia combined with chemotherapy might reserve platinum resistance of COC1/DDP and enhance its sensitivity to cisplatin to some extent.The mechanism may be related to down expression of the survivin mRNA and ERCC1 mRNA.

关 键 词：卵巢癌 COC1 COC1/DDP 顺铂 热疗 Survivin ERCC1 

分 类 号：R71[医药卫生—妇产科学]