分光光度法测定角花胡颓子中的总黄酮  

Determination of total flavonoid in Elaeagnus gonyanthes Benth by spectrophotometry

作　　者：蒋德锡[1] 陈文芝[1] 贾建静[1] 张丹[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2011年第1期81-82,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的采用分光光度法测定角花胡颓子中总黄酮的含量。方法以芦丁为对照品,三氯化铝显色,在417 nm测定吸光度,计算角花胡颓子中总黄酮的含量。结果芦丁4.4～35.2μg.mL-1与吸光度值呈良好的线性关系,回归方程为:Y=0.043X+0.0008(r=0.9999),平均加样回收率为96.21%,RSD=1.39%(n=9)。结论所建方法简便、快速、准确,可用于角花胡颓子的质量控制。OBJECTIVE To establish a spectrophotometric method for determination of total flavonoids in Elaeagnus gonyanthes Benth.METHODS Selecting rutin as control article,colorating with A1C13,a spectrophotometric method was adopted to determine the contents of flavonoids.The total flavonoids in Elaeagnus gonyanthes Benth were calculated.RESULTS The linear concentration range of total flavonoids was 4.4-35.2 μg·mL-1（r=0.9999）and the average recovery of total flavoniods was 96.21% with RSD of 1.39%（n=9）.CONCLUSION This method is simple,quick,accurate and can be used for quality control of Elaeagnus gonyanthes Benth.

关 键 词：角花胡颓子 总黄酮 芦丁 分光光度法 含量测定 

分 类 号：R917[医药卫生—药物分析学]