刚地弓形虫排泄-分泌抗原和可溶性速殖子抗原免疫原性的观察  

Observation on immunogenicity of Toxoplasma gondii excreted/ secreted antigen and soluble tachyzoite antigen

作　　者：刘娟娟[1] 殷国荣[1] 刘红丽[1] 孟晓丽[1] 

机构地区：[1]山西医科大学寄生虫学教研室,医学寄生虫学研究所,太原030001

出　　处：《国际医学寄生虫病杂志》2011年第1期1-6,共6页International JOurnal of Medical Parasitic Diseases

基　　金：国家自然科学基金项目（30640057）

摘　　要：目的观察刚地弓形虫速殖子排泄-分泌抗原（excreted／secreted antigen，ESA）和可溶性速殖子抗原（soluble tachyzoite antigen，STAg）鼻内免疫小鼠的免疫原性。方法BALB／c小鼠随机分为3组，每组10只，分别用PBS20μl/只、ESA和STAg各20μg/只鼻内免疫2次，间隔14d。分别于末次免疫后14d每组处死小鼠，计数肠上皮内淋巴细胞（intestinal intraepithelial lymphocytes，iIEL）和脾淋巴细胞，ELISA法检测血清IgG和小肠冲洗液IgA抗体水平。结果实验期间，末次免疫后14d，各抗原组脾淋巴细胞及iIEL均增殖活跃，细胞数与PBS组比较，差异具有统计学意义（P〈0．05）。各抗原组血清IgG水平在免疫后14d明显增高，与PBS组比较差异有统计学意义（P〈0．01）。免疫后14d肠液IgA水平ESA和STAg组与PBS组比较差异有统计学意义（P〈0．01）。结论ESA和STAg鼻内免疫均可诱导黏膜及系统的细胞和体液免疫应答，有较强的免疫原性。Objective To study the immunogenicity of Toxoplasma gondii excreted/secreted antigens （ESA） and soluble tachyzoite antigen （STAg） after intranasal immunization. Methods BALB/e mice were randomly divided into 3 groups, 10 mice each group. Mice were intranasally immunized with 20 ~1 PBS per mouse and 20 p.g ESA or STAg per mouse, respectively, twice at an internal of 2 weeks. Ten mice of each group were killed on day 14 after the last immunization. Spleen lymphocytes and intestinal intraepithelial lymphocytes （iIEL） were counted. Serum IgG and IgA in intestinal washes were detected by ELISA. Results Cell proliferation of spleen lymphocytes and iIEL was observed in all antigen groups on day 14 after the last immunization, and compared with the PBS group, there was a significant difference （ P 〈 0.05 ）. The level of serum IgG in all antigen groups was higher than that in PBS group on day 14 after immunization with a significant difference （P 〈0.01 ）. On day 14 after immunization, the level of IgA in ESA group and STAg group was higher than that in PBS group （ P 〈 0.01 ）. Conclusion Intranasal immunized with ESA or STAg can effectively induce the mucosal and systemic immune responses with potent immunogenicity.

关 键 词：刚地弓形虫 排泄-分泌抗原 可溶性速殖子抗原 鼻内免疫 免疫原性 

分 类 号：R382.5[医药卫生—医学寄生虫学]