人体蠕形螨基因组DNA提取方法的比较研究  

Comparison between two methods of extracting DNA from Demodex folliculorum

作　　者：佘俊萍[1] 张锡林[2] 王光西[1] 杨燕[1] 何谐[2] 王利芳[1] 

机构地区：[1]四川泸州医学院病原生物学教研室,646000 [2]重庆第三军医大学病原生物学教研室,400038

出　　处：《国际医学寄生虫病杂志》2011年第1期7-9,共3页International JOurnal of Medical Parasitic Diseases

基　　金：2011年四川省卫生厅资助课题（100245）及泸州医学院学院2010年资助课题（767）

摘　　要：目的比较两种人体蠕形螨基因组DNA提取方法，用随机扩增多态性DNA（random amplified polymorphicDNA，RAPD）方法进行初步分析。方法分别用经典方法和小型昆虫试剂盒提取人体蠕形螨基因组DNA，并设计5条随机引物，应用RAPD技术对人体毛囊蠕形螨基因组DNA进行随机扩增，扩增产物经1．5％琼脂糖凝胶电泳后分析。结果小型昆虫试剂盒提取人体蠕形螨基因组DNA的纯度高于酚氯仿抽提方法，但浓度低。引物P3扩增出2条人体蠕形螨条带。引物P4扩增出3条条带。结论经典方法提取人体蠕形螨基因组的DNA能够满足基因组DNA的RAPD分析要求。Objective To compare two methods of extracting DNA from Demodex folliculorum and to preliminarily analyse DNA with RAPD. Methods DNA of follicle mites was extracted by either phenol-chlor- oform extraction or insect DNA extracting kit. Five pairs of random primers were designed to randomly amplify the genomic DNA by random amplified polynmrphic DNA（RAPD）. The products were analyzed by 1.5% agarose electrophoresis. Results The genomic DNA of demodicid mites extracted with insect DNA kit was more pure than DNA extracted by phenol-chloroform extraction, but its concentration was much lower than that ex- tracted by phenol-chloroform extraction. Two and 3 bands were obtained with P3 and P4 primers, respectively. Conclusion The genomic DNA extracted by phenol-chloroform extraction could meet the requirement of RAPD analysis.

关 键 词：毛囊蠕形螨 DNA抽提 随机扩增多态性DNA 

分 类 号：R512.62[医药卫生—内科学]