铜绿假单胞菌生物被膜的形成与环境氧浓度变化的关系研究  

Biofilm production of Pseudomonas aeruginosa under different levels of environmental oxygen

作　　者：刘立坤[1] 吕晓娟 李永乾[1] 

机构地区：[1]河北医科大学第三医院输血科,石家庄050051 [2]赵县仁济医院内科,石家庄050000

出　　处：《国际呼吸杂志》2011年第3期187-189,共3页International Journal of Respiration

摘　　要：目的观察氧浓度变化对铜绿假单胞菌生物被膜产生量的影响，对存在铜绿假单胞菌感染高危因素的患者是否适合高浓度的氧疗做出分析。方法10株铜绿假单胞菌临床分离株（均来源于不同克隆株）分别在无氧（0％）、低氧（10％）、正常氧（20％）、高氧（30％、40％、50％、60％）7个不同的氧浓度下连续培养3d后，分别用结晶紫染色法检测生物被膜产生量；硝酸银染色法观察不同氧浓度环境下生物被膜的重要成分胞外多糖产生的时间窗。结果氧浓度为50％时生物被膜产生量达到最高值（A570=1．61），趋势性分析表明其产生量均随着氧浓度的升高而上升，相关性分析显示其产生量与氧浓度呈正相关（R=0．59）。胞外多糖的检测结果显示氧浓度大于40％时，菌株培养1h镜下即可见到银染成黑色的胞外多糖；氧浓度为20％、30％时，所需时间为2h；10％氧浓度需要4h；无氧条件下需要8h，即随着氧浓度的升高细菌出现胞外多糖的时间窗越短。结论体外实验中铜绿假单胞菌在高氧环境下更容易产生生物被膜。Objective The aims of this study were to investigate the relationship between oxygen concentration and biofilm production of P. aeruginosa. Methods Ten genetically different clinical strains of P. aeruginosa were cultured for 3 days at different levels of environmental oxygen such as no oxygen （0%）, low oxygen （10%）, normal oxygen （20%） and high oxygen （30%, 40%, 50%, 60%）, respcetively. Then biofilm mass was quantified by crystal violet dye, silver nitrate staning to determine the time window of extra polysaccharide production under different levels of environmental oxygen. Results For quantifying the amount of biofilm mass, the highest amount of biofilm mass （1. 61） was produced when the oxygen concentration was 50%. Linear correlation analysis indicated that a significant positive correlation existed between the oxygen concentration and biofilm mass produetion（ R= 0.59）. The production of extra polysaceharide by P. aeruginosa was not visualized until culture for 1 hour at 〉40% environmental oxygen coneentration, 2 hours for 20% and 30 % oxygen concentrations, 4 hours for 10 oxygen concentration, and 8 hours for anaerobic conditions. The time window of extra polysaccharide production was shorter with the increase of oxygen. Conclusions Hyperoxia promoted the ability of producing biofilm of P. aeruginosa which is in favor of resistance and colonization.

关 键 词：铜绿假单胞菌 氧浓度 生物被膜 

分 类 号：R446.5[医药卫生—诊断学]