La_(0.7)Sr_(0.3)NiO_3/壳聚糖/聚吡咯/牛血红蛋白修饰玻碳电极的制备及构建过氧化氢传感器  

Preparation of La_(0.7)Sr_(0.3)NiO_3/chitosan/PPY/Hb modified glassy carbon electrode and it use as hydrogen peroxide biosensor

作　　者：储艳文[1] 林生岭[1] 郑佩[1] 郭寅丹[1] 龙丹[1] 万勇[1] 

机构地区：[1]江苏科技大学材料科学与工程学院,江苏镇江212003

出　　处：《功能材料》2011年第B02期47-49,61,共4页Journal of Functional Materials

基　　金：国家自然科学基金资助项目(20704020);江苏科技大学博士启动基金资助项目(2006CL0035)

摘　　要：在玻碳电极上修饰一层表面均匀的聚吡咯膜，然后利用钙钛矿和壳聚糖的复合膜来固定牛血红蛋白（Hb），制备出性能良好的过氧化氢生物传感器。采用循环伏安（CV）和扫描电镜对修饰电极进行了表征。该传感器对H2O2具有好的催化响应，且响应快。在优化的实验条件下，所制备的传感器对H2O2的线性范围为7．0×10^-6～1．5×10^-3mol／L，检测限为9．0×10^-8mol／L．A uniform film of polypyrrole was electrochemically polymerized and immobilized on the surface of glassy carbon electrode（GCE）, and a layer of La0.7SrO3NiO3 and chitosan（CS） composite film was absorbed on the surface of PPY film. Preparation of the enzyme modified electrode , used as biosensor for determination of H202,was finally completed by immobilization of the bovine hemoglobin（Hb） on to the surface of La0.7Sr0.3 NiO3/CS/GCE. The modified electrode were characterized with cyclic voltammetry （CV） and scanning electron microscope （SEM）. The biosensor displays excellent catalytic activity and rapid response for H2O2. The linear range for the determination of H2O2 is from 7.0×10^-6～1.5×10^-3mol/L with a detection limit of 9.0×10^-8mol/L

关 键 词：聚吡咯 La0.7Sr0.3NiO3 壳聚糖 牛血红蛋白 过氧化氢传感器 

分 类 号：O657.1[理学—分析化学]