中国甜菜坏死黄脉病毒RNA5的检测及其核苷酸序列分析  

Detection and Nucleotide Sequence Analysis of Beet Necrotic Yellow Vein Virus RNA5 Isolated from ChinaProject Granted by Chinese National Natural Science Fund (No.39670035 and 39770492). The Nucleotide Sequence Data Reported in this Paper have be

作　　者：李大伟[1] 于嘉林[1] 韩成贵[1] 刘涛[1] 秦树才 刘仪[1] RenateKoenig 

机构地区：[1]中国农业大学农业生物技术国家重点实验室,北京100094 [2]BiologischeBundesastaltfurLandundForstwirtschaft

出　　处：《生物工程学报》1999年第4期461-465,共5页Chinese Journal of Biotechnology

基　　金：国家自然科学基金

摘　　要：利用反转录 Ｐ Ｃ Ｒ 方法，对甜菜坏死黄脉病毒（ Ｂ Ｎ Ｙ Ｖ Ｖ）５ 个中国分离物的 Ｒ Ｎ Ａ５进行了检测，结果表明新疆分离物、黑龙江分离物和内蒙古乌拉特前旗分离物不含有 Ｒ Ｎ Ａ５ ，只有包头分离物和呼和浩特分离物有 Ｒ Ｎ Ａ５ 。序列分析结果，包头分离物和呼和浩特分离物 Ｒ Ｎ Ａ５ 基因组分别为１３３８ ｎｔ 和１３５８ ｎｔ，均只包含１ 个开放阅读框架，编码产生２６ ｋ Ｄ 蛋白。与法国 Ｆ７２ 分离物和日本 Ｄ５ 分离物相比， 各分离物间核苷酸序列同源性为９３７ ％ ～９８５ ％ ，由此推导的氨基酸序列同源性为９１８ ％ ～９８２ ％ 。Using RT PCR method,the RNA5 genomic component was detected for five beet necrotic yellow vein virus (BNYVV) isolates from China. The result shows that RNA5 was only found in Baotou isolate and Hohhot isolate,but not in Xingjiang isolate,Helongjiang isolate and Wulate isolate.Nucleotide sequence analysis shows that the RNA5 genome of Baotou isolate and Hohhot isolate has 1338nt and 1358nt in length respectively,which contain single open reading frame (ORF) encode protein with molecular weights of 26kD. Comparing with the published sequences of F72 and D5 isolates,they share 93.7%～98.5% nucleotide identity and 91.8%～98.2% amino acid identity each other.

关 键 词：甜菜 坏死黄脉病毒 RNA5基因组 检测 序列分析 

分 类 号：S432.41[农业科学—植物病理学]