几种真菌诱导子对云南红豆杉细胞产生紫杉醇的影响  

Effects of Fungus Elicitors on Taxol Production in Suspension Cells of Taxus yunnanensis

作　　者：陈永勤[1] 朱蔚华[1] 吴蕴祺 胡秋[1] 

机构地区：[1]中国医学科学院中国协和医科大学药物研究所,北京100050

出　　处：《生物工程学报》1999年第4期522-524,共3页Chinese Journal of Biotechnology

摘　　要：The effects of the cultures of Penicilliun citrinum,Absidia glauca,Mucor rouxianus and Botrytis cinerea on the growth and taxol production in suspension cells of Taxus yunnanensis were investigated.The results showed that the addition of the cultures of Penicilliun citrinum or Botrytis cinerea into the suspension cell cultures of T.yunnanensis at the final stage of fast growing phase(the 12th day of culture) did not inhibited the cell growth,but dramatically increased taxol yield;The best result was obtained in the elicitation with Penicilliun citrinum (3.1 g/L dry mycelium) for 3 days,in which the taxol yield was increased by 1～2 times.The effects of the cultures of Penicilliun citrinum,Absidia glauca,Mucor rouxianus and Botrytis cinerea on the growth and taxol production in suspension cells of Taxus yunnanensis were investigated.The results showed that the addition of the cultures of Penicilliun citrinum or Botrytis cinerea into the suspension cell cultures of T.yunnanensis at the final stage of fast growing phase(the 12th day of culture) did not inhibited the cell growth,but dramatically increased taxol yield;The best result was obtained in the elicitation with Penicilliun citrinum (3.1 g/L dry mycelium) for 3 days,in which the taxol yield was increased by 1～2 times.

关 键 词：云南红豆杉 悬浮细胞培养 真菌诱导子 紫杉醇 

分 类 号：S567.190.3[农业科学—中草药栽培]