豌豆热激蛋白Hpc60研究  

Study on the Heat Shock Protein—Hpc60 from Pea Leaf Cytosol 

作　　者：陈忠[1] 苏维埃[1] 汤章城[1] 

机构地区：[1]中国科学院上海植物生理研究所,上海200032

出　　处：《Acta Botanica Sinica》1999年第10期1090-1093,共4页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金!重点资助项目 (No .3 973 0 0 5 0 )

摘　　要：研究了PEG、NaCl以及作为干旱和盐胁迫响应中信号物质的ABA对豌豆(PisumsativumL.)幼苗中一种热激诱导蛋白———Hpc60表达的影响。结果显示PEG可以提高蛋白的表达水平,其最强诱导能力与38℃热激相近。但NaCl在造成植物同等程度水势下降的情况下对此蛋白没有诱导作用,而且,短时间内还不同程度地降低Hpc60蛋白含量。除引起植株水势的变化外,NaCl处理还显著地改变了胞内Na+和Ca2+的含量。而PEG胁迫后K+、Na+和Ca2+均无明显变化。推测造成PEG和NaCl诱导能力差异的原因可能是NaCl和PEG胁迫伤害的机理不同。NaCl胁迫导致Na+的大量内流,破坏了胞内离子平衡。另外,外源施加ABA不影响此蛋白的含量,推测热激和PEG胁迫对此蛋白的诱导过程不需要ABA中介。The effects of NaCl, PEG and exogenous ABA on the expression of the heat shock protein—Hpc60 from pea (Pisum sativum L.) were investigated. The results showed that PEG elevated the quantity of this heat_shock protein, much similar to heat_shock stress. Although NaCl was able to reduce water potential at the same extent with or even higher than PEG, but it did not increase the level of this protein. This phenomenon was closely correlated with the variations of cytoplasmic ion levels. The authors postulated that NaCl treatment caused the influx of Na +, thus disrupted the ion homeostasis, whereas PEG treatment did not. So a rather stable Na +/K + ratio was sustained in the plant cell treated with PEG, which might be prerequisite for the induction of Hpc60. In addition, exogenous ABA was employed in the authors' experiment which showed no influence on the synthesis of this heat_shock protein.

关 键 词：豌豆 热激蛋白 干旱 盐胁迫 

分 类 号：Q948.11[生物学—植物学] Q946.1