重组人睫状神经营养因子的复性研究  

Study of Renaturation of Recombinant Human Ciliary Neurotrophic Factor Expressed in E.coli.

作　　者：咸海青[1] 范明[1] 吴燕[1] 邱宗荫 

机构地区：[1]军事医学科学院基础医学研究所,北京100850

出　　处：《生物化学与生物物理进展》1999年第5期454-457,共4页Progress In Biochemistry and Biophysics

基　　金：国家"863"项目

摘　　要：利用凝胶层析对人睫状神经营养因子原核基因工程产品进行复性研究， 发现此方法的复性率高于稀释透析法． 而且在复性的同时也进行了一步纯化工作． 该方法简单、迅速、高效、重复性好， 可用于规模生产．Renaturation of human cilinary neurotrophic factor expressed in recombinant bacteria , by gel filitration chromatograph, was studied . It was showed that the renaturation rate by this way was higher than that by dilution and dialysis, and the protein was purified at the same time. It was a simple, rapid, good reproducibility and efficient procedure which can be used to produce ciliary neurotrophic factor in large scale.

关 键 词：重组人睫状神经营养因子 复性 包涵体 

分 类 号：Q422[生物学—神经生物学] Q784[生物学—生理学]