确证TRFIA检测乙肝病毒表面抗体结果的研究  

Detection of Hepatitis B Surface Antigen and Antibody by TRFIA

作　　者：吉飞跃[1] 钱开成[1] 

机构地区：[1]南通大学附属海安医院检验科,江苏南通226600

出　　处：《标记免疫分析与临床》2011年第1期33-35,共3页Labeled Immunoassays and Clinical Medicine

摘　　要：建立简易时间分辨免疫荧光法(TRFIA)检测乙型肝炎病毒表面抗体(抗-HBs)的中和试验方法,验证TRFIA检测结果。收集乙型肝炎病毒表面抗原(HBsAg)异常值血清,确定TRFIA中和抑制试验中和比为0.25-1.26ng/mL∶1m IU/mL,对抗-HBs的定量结果进行确认。结果显示,使用TRFIA定量检测的结果与中和抑制试验的结果符合率达100%。结论:使用中和抑制试验确认TRFIA定量检测乙型肝炎病毒(HBV)表面抗体的检测结果,可以排除非特异反应导致的结果。To establish a simple neutralization test to detect hepatitis B surface antigen and antibody（anti-HBs） by TRFIA.The serum samples with outlier serum of heptatitis B surface antigen（HBsAg） were collected,and the neutralization inhibition test was carried out to confirm the quantitative result of anti-HBs which analysis by TRFIA.The results showed that the coincidence rate of anti-HBs analysis by TRFIA quantitative test and neutralization inhibition test was 100%.The neutralization inhibition test could be used to confirm the detection results of hepatitis B surface antigen and antibody by TRFIA and to exclude the result of non-specific reaction.

关 键 词：乙型肝炎病毒表面抗体 中和抑制试验 时间分辨免疫荧光法 

分 类 号：R446.6[医药卫生—诊断学]