人红细胞带3蛋白胞质段的克隆及其表达  

Cloning and Expression of the Cytoplasmic Domain of Human Erythrocyte Band 3 Protein.

作　　者：李至[1] 彭锋[1] 季朝能[1] 宦春雷[1] 张志鸿[1] 

机构地区：[1]复旦大学生命科学学院,立人实验室,上海200433

出　　处：《生物化学与生物物理进展》1999年第5期492-494,共3页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金

摘　　要：人红细胞带３ 蛋白胞质段（ｃｙｔｏｐｌａｓｍｉｃｄｏｍａｉｎ ｏｆ ｂａｎｄ ３，ｃｄｂ３） 起着将膜与膜骨架、细胞内环境相联系的重要作用．以带３ 蛋白全长基因为模板， 用ＰＣＲ方法扩增出ｃｄｂ３ 片段， 克隆至ｐＲＳＥＴ表达质粒上， 转化大肠杆菌ＢＬ２１ （ＤＥ３） ． 转化菌经诱导表达出较高含量的ｃｄｂ３ 蛋白， 纯化后，The cytoplasmic domain of human erythrocyte band 3 (cdb3) plays an important role in connection of the membrane with the membrane skeleton, and environment inside the cell as well. With whole length of band 3 gene, cdb3 gene was amplified by PCR and then was cloned into pRSET plasmid. The plasmid was introduced into E.coli BL21(DE3). The cdb3 expression proteins were purified and its function was verified according to the ability to inhibit aldolase.

关 键 词：带3蛋白 胞质段 质粒 表达 红细胞 

分 类 号：Q513[生物学—生物化学] Q592.1