睫状神经营养因子对NO引起海马神经元毒性反应的影响  

CILIARY NEUROTROPHIC FACTOR PREVENTS NO MEDIATED CYTOTOXICITY IN CULTURED HIPPOCAMPAL NEURONS

作　　者：陈秀青[1] online.sh.cn 陈哲宇[1] 路长林[1] 何成[1] 王成海[1] 鲍璿[2] 

机构地区：[1]第二军医大学神经科学研究所神经生物学教研室 [2]中国科学院上海脑研究所,上海200031

出　　处：《生理学报》1999年第5期501-507,共7页Acta Physiologica Sinica

摘　　要：本研究采用原代培养大鼠海马神经元，观察睫状神经营养因子（ciliaryneurotrophicfactor，CNTF）对NO引起细胞毒性反应的影响。NO供体硝普钠与S-亚硝基-乙酰青霉胺，NOS底物L-Arg及钙载体ionomycin，均可引起海马神经元存活率下降，LDH漏出增加；提前24h给予不同浓度CNTF，均能提高神经元的存活率，减少LDH漏出，其作用呈剂量依赖性；CNTF可降低ionomyin引起的NO含量升高；CNTF对神经元存活率的影响与NO含量呈显著负相关。上述结果表明，CNTF能抑制NO引起海马神经元的损伤。The effects of ciliary neurotrophic factor on nitric oxide (NO)-induced cytotoxicitywere examined in primary cultures of rat hippocampal neurons. The neurotoxicity wasinduced by NO donors, such as sodium nitroprusside (SNP ), s-nitroso-Nacetylpenicillamine (SNAP), NOS substrate, L-arginine (L-Arg) and ionomycin (acalcium ionophore ). Dreincubation of the cultures with ciliary neurotrophic factor(CNTF) for 24 h promoted nearonal survival and decreased the LDH efflux and the levelof NO induced by ionomycin. The protective effects of CNTF against NO cytotoxicity wereconcentration-dependent. The above findings suggest that CNTF promotes the survival ofneurons in the hippocampus by preventing NO mediated neurotoxicity.

关 键 词：睫状神经 营养因子 海马神经元 NO 细胞毒性 

分 类 号：Q422[生物学—神经生物学] R361.3[生物学—生理学]