热休克蛋白、蛋白水解酶和磷酸酶在大鼠肝再生中的含量和活性变化  

CHANGES IN THE CONTENT AND ACTIVITY OF HSC70/HSP68, PROTEINASES AND PHOSPHATASES DURING LIVER REGENERATION

作　　者：徐存拴 public.xxptt.ha.cn 夏民[1] 卢爱灵[1] 李效阳 李永辉[1] 赵绪永[1] 胡轶红[1] 

机构地区：[1]河南师范大学生命科学学院

出　　处：《生理学报》1999年第5期548-556,共9页Acta Physiologica Sinica

基　　金：河南省生物工程重点实验室资助

摘　　要：本文以2／3肝切除（partialhepatectomy，PH）大鼠为模型，探讨了PH后酸性和碱性磷酸酶（acidandalkalinephosphatases，ACP和AKP）、构成性热休克蛋白70／诱导性热休克蛋白68（HSC70／HSP68）、酸性和中性蛋白水解酶在肝再生期间（0～144h）的动态变化。结果显示，在肝切除后的肝再生期间：（1）ACP和AKP均出现两个活性高峰（4和48h、16和96h），在每个活性高峰之后，ACP和AKP活性均有显著下降；（2）HSC70/HSP68在16和96h时出现两个含量高峰，第二个高峰后的最低点（接近于对照）比第一个高峰后的最低点低许多；（3）在2／3肝切除后2－6h时，诱导出一个90kD的中性蛋白水解酶；36h时，诱导出一个27kD的酸性蛋白水解酶。结果提示，ACP和90kD中性蛋白水解酶可能参与激活G0期肝细胞进入G1期；AKP和HSC70／HSP68可能主要在DNA合成、细胞代谢和增殖方面起作用；27kD酸性蛋白水解酶可能在肝细胞再分化和肝组织重建中有一定作用。This article reports changes in acid and alkaline phosphatases (ACP and AKP),conshtutive heat shock protein 70/induced heat shock protein 68 (HSC70/HSP68) andacid and neutal proteinases ddring liver regeneration (0 ~ 144 h) after 2/3 hepatectomy(pwhal hepatectomy, PH). Both ACP and AKP had two achve peaks at 4 and 48 h, 16and 96 h, respectively, which were followed by sighficant decrease. The content ofHSC70/HSP68 also showed two Peaks (16 and 96 h), of which the content after thesecond Peak decnaed more obviously than after the first peak. Moreover a 90 kD neutalproteinase was induced at the time from 2 to 6 h and a 27 kD acid proteinase was inducedat 36 h. The results suggest that the ACP and the 90 kD neuhal proteinase rnayparticipate in activahng hepatocytes from G0-Phase into G1-phase, and are and HSC70/HSP68 may play a role ndnly in DNA synthesis, cellular metabolism and proliferation.Fdriermore 27 kD acid proteinase may be involved in re-differentiation of hepatocytesand reconsmichon of liver tissue.

关 键 词：肝再生 热休蛋白 蛋白水解酶 复性电泳 

分 类 号：R339.37[医药卫生—人体生理学]