RP-HPLC法测定水黄皮药材中水黄皮素的含量  

RP-HPLC determination of Karanjin in Pongamia pinnata

作　　者：赵映淑[1] 朱毅[1] 陈国彪[1] 

机构地区：[1]海南省药品检验所,海南省药物质量研究重点实验室,海口570216

出　　处：《药物分析杂志》2010年第12期2287-2289,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：国家科技支撑计划课题(No.2007BAI27B06);海南省重点科技项目(NO.070205)

摘　　要：目的：建立RP-HPLC测定水黄皮药材中水黄皮素含量的方法。方法：以Hypersil-C18ODS2（4.6mm×150mm,5μm）为色谱柱,0.1%甲酸溶液（三乙胺调pH=3.0）-乙腈（60∶40）为流动相,流速0.8mL·min-1,检测波长258nm,柱温35℃。结果：水黄皮素进样量在0.116~0.696μg范围内线性良好（r=0.9999,n=6）,平均回收率（n=9）为99.3%。结论：本方法准确、简便,重复性好,可用于水黄皮药材中水黄皮素的含量测定。Objective：To establish an HPLC method for the determination of karanjin content in Pongamia Pinnata by RP-HPLC.Methods：A Hypersil-C18ODS 2（4.6 mm×150 mm,5 μm）column was used with a mobile phase of 0.1% methanoic acid solution（pH was adjusted to 3.0 with triethylamine）-acetonitrile（60∶40）.The flow rate was 0.8 mL·min-1and the column temperature was set at 35 ℃.The detection wavelength was 258 nm.Results：The linear range of karanjin was 0.116-0.696 μg（r=0.9999,n=6）,and the average recovery（n=9）was 99.3%.Conclusion：The method is accurate,sample,reproducible,and can be used to determine the content of karanjin in P.pinnata.

关 键 词：水黄皮 水黄皮素 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]