ELISA检测疫苗中卡那霉素残留量的实用性验证  

The practicability verification of ELISA detect kanamycin residue in vaccines

作　　者：姜崴[1] 庄茂欣[1] 褚东琳[1] 徐文[1] 张宁[1] 赵小琳[1] 郑晓丽[1] 

机构地区：[1]长春生物制品研究所,长春130062

出　　处：《药物分析杂志》2010年第12期2422-2424,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:验证ELISA检测疫苗中卡那霉素残留量的实用性。方法:采用间接竞争ELISA方法检测卡那霉素,并对方法的回收率、线性、特异性、精密性、耐用性等进行验证。验证方法有效后对相关样品进行检测。结果:该方法的回收率达到90%,对卡那霉素的有效检测范围为0.5～40.5ng·mL-1,标准曲线相关系数r=0.9996,RSD小于10%。结论:此方法可以用于检测疫苗中卡那霉素残留量。Objective：To verify ELISA detects kanamycin residue detection in vaccines.Methods：Use indirect competitive ELISA method to detect kanamycin,and verify the method＇s recovery,linearity,specificity,precision,durability.After confirm the method is effective,detect the relevant samples.Results：The recovey of the method was 82%,the results of the effective detection range for kanamycin was 0.5-40.5 ng·mL-1,standard curve correlation coefficient r=0.9996,the RSD of variation was less than 10%.Conclusion：The method can be used to detect vaccines kanamycin residues.

关 键 词：疫苗 卡那霉素 

分 类 号：R917[医药卫生—药物分析学]