腹腔注射沙利度胺对骨癌痛小鼠痛行为的影响  

Effect of intraperitoneal injection of thalidomide on pain behaviors in a mouse model of bone cancer pain

作　　者：郑亚国[1] 马正良[1] 梅凤美[1] 张睿[1] 任炳旭[1] 张娟[1] 顾小萍[1] 

机构地区：[1]南京大学医学院附属鼓楼医院麻醉科,南京210008

出　　处：《中华行为医学与脑科学杂志》2010年第11期1005-1007,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：江苏省＂兴卫工程＂医学重点人才课题资助项目（RC2007012）;卫生部资助项目（WKJ2006-2-8）

摘　　要：目的 观察腹腔注射沙利度胺对骨癌痛小鼠痛行为的影响.方法 36只C3H/HeJ小鼠随机分为肿瘤组（n=18）和假手术组（n=18）,每组抽取6只小鼠用于观察肿瘤细胞接种后小鼠痛行为学的变化.将含2×105个纤维肉瘤NCTC2472细胞的最小必需培养基（α-MEM）注射到小鼠右侧股骨远端骨髓腔内,制作骨癌痛模型,假手术组注入不含肿瘤细胞的α-MEM.在接种前1 d,肿瘤组再分为两组（n=6）：肿瘤＋Thalidomide组,肿瘤＋Vehicle组;假手术组相应分为假手术＋Thalidomide组,假手术＋Vehicle组.肿瘤＋Thalidomide组和假手术＋Thalidomide组在术后第1天到第7天,每天腹腔注射50 mg/kg的沙利度胺;肿瘤＋Vehicle组和假手术＋Vehicle组于同一时间注射等量体积的溶剂.各组于接种前1 d,接种后第3天、5天、7天、10天、14天检测痛行为学指标,包括机械缩足阈值（PWMT）和热缩足潜伏期（PWTL）.结果 （1）接种肿瘤细胞后第7天,肿瘤组小鼠PWMT（1.07±0.30）g与假手术组（1.70±0.33）g相比,显著降低（P＜0.05）;接种后第10天,肿瘤小鼠的PWTL（12.60±1.69）s较假手术小鼠（17.70±1.54）s明显缩短（P＜0.05）,肿瘤小鼠的痛行为学随时间逐渐加重;（2）接种肿瘤细胞后第7天,肿瘤＋Thalidomide组小鼠PWMT（1.53±0.39）g与肿瘤＋Vehicle组（1.07±0.39）g相比,显著升高（P＜0.05）;接种后第10天,肿瘤＋Thalidomide组小鼠的PWTL（16.48±1.13）s较肿瘤＋Vehicle组小鼠（12.64±1.56）s明显延长（P＜0.05）.结论 腹腔注射沙利度胺能够显著改善骨癌痛小鼠的痛行为.Objective To investigate the effect of intraperitoneal injection of thalidomide on pain behaviors in a mouse model of bone cancer pain. Methods 36 male C3H/HeJ mice were divided randomly into tumor group （n= 18） and sham group （n= 18） ,six mice from each group were chosen to examine the time course of changes in behavior after tumor cells inoculated to the bone. 2 × 105 osteosarcoma NCTC 2472 cells were implanted into the intramedullary space of the right femurs of mice to induce ongoing bone cancer related pain behaviors. The sham group was inoculated by α-MEM without any cells. On the day before inoculation,the tumor mice were divided randomly into tumor ＋ thalidomide group and tumor ＋ vehicle group. The sham group mice were further divided randomly into sham ＋ thalidomide group and sham ＋ vehicle group. Pain ethology indexes such as paw withdrawal mechanical threshold （PWMT） and paw withdrawal thermal latency （PWTL） were observed on 1 d before inoculation and on 3 d ,5 d ,7 d, 10 d, 14 d after inoculation. Results （ 1 ） At day 7 after the operation, compared with sham mice （ 1. 70 ± 0. 33 ） g, PWMT of tumor mice decreased to （ 1.07 ± 0. 30） g （P 〈 0. 05 ）. At day 10, PWTL shortened to （ 12.60 ± 1.69 ） s （P 〈 0. 05 ） compared with sham mice （ 17.70 ± 1.54 ） s. And the pain behaviors of tumor mice were aggravated along with the development of cancer pain. （2） At day 7 after the operation, compared with tumor ＋ vehicle group （ 1. 07 ± 0.39 ） g, PWMT of tumor ＋ thalidomide group increased to （ 1. 53 ± 0. 39 ） g （P 〈0.05）. At day 10, PWTL extended to （ 16.48 ± 1.13 ） s compared with sham mice （ 12.64 ± 1. 56） s （P 〈0. 05 ）. Conclusion Intraperitoneal injection of thalidomide can efficiently relieve mechanical hyperalgia and thermal hyperalgia in a mouse model of bone cancer pain.

关 键 词：沙利度胺 骨癌痛 机械痛敏 热痛敏 

分 类 号：R965[医药卫生—药理学]