牙鲆dmrt1基因的克隆及其与P450arom基因的组织表达分析  

Cloning of dmrt1 gene and its tissue expression analyses compared with that of P450arom gene in olive flounder (Paralichthys olivaceus)

作　　者：文爱韵[1,2] 尤锋[1] 孙鹏[1] 徐冬冬[1] 吴志昊[1] 马得友[1] 李军[1] 张培军[1] 

机构地区：[1]中国科学院海洋研究所中国科学院实验海洋生物学重点实验室,山东青岛266071 [2]中国科学院研究生院,北京100039

出　　处：《海洋科学》2010年第11期97-102,共6页Marine Sciences

基　　金：国家973项目(2010CB126304);国家863计划资助项目(2006AA10A404)

摘　　要：通过基因组步移和3’RACE获得了牙鲆(Paralichthys olivaceus)dmrt1的cDNA全序列,该基因开放阅读框全长909bp,其编码的蛋白具有高度保守的DM结构域,5’UTR区含有性别相关转录因子Sox9和Sox5的结合位点。牙鲆dmrt1基因只在牙鲆的性腺中表达,且在精巢中的表达明显高于卵巢,表明牙鲆的dmrt1可能是一种性别相关基因。同时,对牙鲆的另一性别相关基因P450arom在成体各组织的表达分析结果表明,该基因除在牙鲆的性腺中有表达外,在肾脏、脾脏、鳃和脑等其他组织中也有不同程度的表达。牙鲆P450arom基因在性腺中的表达也存在两性差异,其表达模式与dmrt1的正好相反,在卵巢中的表达量明显高于精巢。Whole cDNA sequence of olive flounder（Paralichthys olivaceus） dmrt1 gene was obtained using genome walking and 3＇RACE methodss.The open reading frame of flounder dmrt1 was found to span 909 bp.Sex-related transcriptional factors Sox9 and Sox5 binding sites were identified inin its 5＇UTR.Flounder Dmrt1 protein had a high conserved DM domain.Expressions of dmrt1 and P450arom genes in flounder tissues were also studied by RT-PCR.The results showed that both genes were sex-related genes.The dmrt1 gene was gonad-specific,expressed and was sexual dimorphic expressed with much higher expression in testis and much lower expression in ovary.The expression of P450arom in gonads was totally opposite to that of dmrt1.It was higher expressed in ovary and lower expressed in testis.Moreover,flounder P450arom had a broad-spectrum expression pattern,being that it was also differentially expressed in some other tissues,such as like kidney,spleen,gill and brain.

关 键 词：牙鲆(Paralichthys olivaceus) DMRT1 P450AROM 两性差异表达 

分 类 号：Q75[生物学—分子生物学]